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Table 3.

Fgf8 over-expression is sufficient to induce foxi1-positive EB precursors and phox2b-positive EB neurons1

Bead→hostStage(hpf)/marker2n3Ectopic/rescued
BSA→wt 19/foxi1 
Fgf8b→wt 19/foxi1 15 
BSA→wt 48/phox2b 12 
Fgf8b→wt 48/phox2b 10 
BSA→fgf3+8-MO 19/foxi1 11 
Fgf8b→fgf3+8-MO 19/foxi1 15 12 
Fgf8b→fgf3+8-MO (hindbrain) 19/foxi1 
Bead→hostStage(hpf)/marker2n3Ectopic/rescued
BSA→wt 19/foxi1 
Fgf8b→wt 19/foxi1 15 
BSA→wt 48/phox2b 12 
Fgf8b→wt 48/phox2b 10 
BSA→fgf3+8-MO 19/foxi1 11 
Fgf8b→fgf3+8-MO 19/foxi1 15 12 
Fgf8b→fgf3+8-MO (hindbrain) 19/foxi1 
1

One to three BSA- or Fgf8b-coated beads were implanted into wild-type or fgf3;fgf8 morphants at 11-13 hpf(Fig. 7).

2

Resulting embryos were collected at either 19 or 48 hpf and analyzed for foxi1 or

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