Cephalic mesoderm restoration in fgf3;fgf8 morphants rescues EB ganglia1
| wt→fgf3+8-MO . | n2 . | gVII3 . | gIX . | Small gX . | Large gX . |
|---|---|---|---|---|---|
| Mesoderm alone | 8 | 3 | 1 | 3 | 8 |
| Mesoderm and hindbrain | 7 | 1 | 2 | 2 | 6 |
| Hindbrain alone | 42 | 0 | 0 | 0 | 0 |
| Total rescued | 15 | 4 (14) | 3 (2) | 5 (2) | 14 (4) |
| wt→fgf3+8-MO . | n2 . | gVII3 . | gIX . | Small gX . | Large gX . |
|---|---|---|---|---|---|
| Mesoderm alone | 8 | 3 | 1 | 3 | 8 |
| Mesoderm and hindbrain | 7 | 1 | 2 | 2 | 6 |
| Hindbrain alone | 42 | 0 | 0 | 0 | 0 |
| Total rescued | 15 | 4 (14) | 3 (2) | 5 (2) | 14 (4) |
25-30 donor cells were transplanted into the margin of a host embryo at 30-40% epiboly (Fig. 6);resulting embryos were collected at either 48 or 72 hpf and analyzed for phox2b (wild-type hosts) or EGFP (phox2b::egfp hosts)expression, respectively.
Out of 226 embryos that received the transplant, 15 (7%) displayed rescue of at least one EB ganglion.