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Table 1.

Fgf signaling is required cell-autonomously in EB placodes1

n2 (total)n3 (target)Total number of Pax2 cells in f4 placodeTotal number of Pax2 cells in g and v placodesAverage number of Pax2 donor cells in f placodeAverage number of Pax2donor cells in g and v placodes
wt→wt 176 46 (26%) 44 70 11 13 
hsp70::dn-fgfr1→wt 112 39 (35%) 395 66 16 36 
 n2 (total) n3 (target) Total number of Pax2 cells Average number of Pax2 host cells Average number of Pax2 donor cells  
wt→hsp70::dn-fgfr1 (transplant side) 78 20 (26%) 547 39 15  
wt→hsp70::dn-fgfr1 (contralateral control)   42 42  
n2 (total)n3 (target)Total number of Pax2 cells in f4 placodeTotal number of Pax2 cells in g and v placodesAverage number of Pax2 donor cells in f placodeAverage number of Pax2donor cells in g and v placodes
wt→wt 176 46 (26%) 44 70 11 13 
hsp70::dn-fgfr1→wt 112 39 (35%) 395 66 16 36 
 n2 (total) n3 (target) Total number of Pax2 cells Average number of Pax2 host cells Average number of Pax2 donor cells  
wt→hsp70::dn-fgfr1 (transplant side) 78 20 (26%) 547 39 15  
wt→hsp70::dn-fgfr1 (contralateral control)   42 42  
1

25-30 donor cells were transplanted into the presumptive placodal domain of shield-staged host embryos (Fig. 3); resulting embryos were heat-shocked at 38.5°C for 30 minutes (bud-3s for hsp70::dn-fgfr1→wt transplants and 9-14s for wt→hsp70::dn-fgfr1 transplants), collected at 24 hpf and analyzed for Pax2 expression.

2

Total number of embryos that received transplanted cells.

3

Number of embryos with at least one Pax2-positive donor cell.

4

f, presumptive facial placode; g, presumptive glossopharyngeal placode; v,presumptive vagal placode.

5

P<0.014, t-test.

6

P<0.001, t-test.

7

P<0.004, paired t-test.

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