Gene identities, abbreviations, accession numbers and qRT-PCR primer sequences
| Gene identity (abbreviation) . | Accession no. . | Forward primer (5′-3′) . | Reverse primer (5′-3′) . |
|---|---|---|---|
| Ligand-binding proteins | |||
| 1. Hexamerin-1 (Hex-1) | AY572858 | GATCCATTCCACAAGCACG | ACATTCTCCACCGTCACTCC |
| 2. Hexamerin-2 (Hex-2) | AY572859 | ACGGAAGACGTTGGACTCAG | GAGGACCTGCTGGATCTTGT |
| Cuticle and muscle proteins | |||
| 3. Larval Cuticle Protein A3A-like (LCP) | DN792534 | CGTCGACACCGACTACGAC | GGTCAGCGGTGTACTCGAC |
| 4. Rf1 Troponin-1 [wup-like] (Trop-1) | CB518302 | CGACCTAGAATACGAAGTGG | TTCTTCTCCTTGTCCTCCTCC |
| 5. Rf2 Troponin-1 [wap-like] (Trop-2) | CB518303 | GAAGAGTTGAAGAAGGAGCAGG | TTGTTCACCAGGCTATTCAGG |
| Transcription and translation factors | |||
| 6. nanos | BQ788190 | CCACTGACTAAATGTATGGG | TTCAAGCCTCAACACTCTGT |
| 7. COP9 Complex Homolog Subunit 5 (COP9) | DN792518 | CTCGATCAGGAGGCACACTC | TTGCTGCCTCAATGTATGCT |
| 8. BTB-POZ [broad-like] | AY258590 | CTGGACCAGCATCTACATCTTC | GGTTGAAGCCTGATTCACAAG |
| 9. 18S rRNA-like (18S) | AY572860 | TATCGATCCTTTTGGCTTGG | TCGCAATGATAGGAAGAGCC |
| 10. 28S rRNA-like (28S) | CK906357 | GCGAATGATTAGAGGCCTTG | ACAGCGCCAGTTCTGCTTAC |
| 11. bicaudal (bic) | AY258589 | GAGGCAAGTACGGATTGGAG | CTCTTCATGGTAACAACCAG |
| 12. LIM | CB518301 | GTGCTTCAAGTGTGGCATGT | GTCCATGCTGAGACAACCAG |
| Signal transduction factors | |||
| 13. Malonyl Co-A Decarboxylase (M-CoA) | AY572861 | GCTACCGGCGACTCTTAATC | GAGGACACGCTGATTCCTTC |
| 14. Phospho-Inositol Phosphate (PIP) Kinase | CK906365 | AATTCTGCTGCCTTCTCTGG | ACCTTGCTTGGTCAACCATC |
| 15. Apoptosis Inhibitor (Apop) | CK906364 | CGTACATGTGTGAGCAGGTG | ATCACCATCAGGTGGCAGAG |
| 16. AMP Deaminase (AMP-de) | CK906363 | GTGACATGTGTGAGCTTGCC | ACCAGCTACACCTTCCTTGG |
| 17. GTPase Activating Protein (GAP) | BQ788178 | TTCCAACAGCACAAGAGCAC | TAACTGGTTGCGACAGGCAC |
| 18. ATPase | BQ788171 | TCAGGAAGTCTTGGATTCGG | TACGAACTCTGGTGCGTCTG |
| 19. SH3 Domain Kinase (SH3) | CB518513 | CGTGTTGCCAATGAGTTGAG | ACAATCCTATTCGGCCATCC |
| Reference/control genes | |||
| 20. β-actin | DQ206832 | AGAGGGAAATCGTGCGTGAC | CAATAGTGATGACCTGGCCGT |
| 21. NADH-dh | BQ788175 | GCTGGGGGTGTTATTCATTCCTA | GGCATACCACAAAGAGCAAAA |
| 22. HSP-70 | BQ788164 | AGAACCAAGTGGCCATGAAC | CCAATGCTTCATGTCTGCC |
| Gene identity (abbreviation) . | Accession no. . | Forward primer (5′-3′) . | Reverse primer (5′-3′) . |
|---|---|---|---|
| Ligand-binding proteins | |||
| 1. Hexamerin-1 (Hex-1) | AY572858 | GATCCATTCCACAAGCACG | ACATTCTCCACCGTCACTCC |
| 2. Hexamerin-2 (Hex-2) | AY572859 | ACGGAAGACGTTGGACTCAG | GAGGACCTGCTGGATCTTGT |
| Cuticle and muscle proteins | |||
| 3. Larval Cuticle Protein A3A-like (LCP) | DN792534 | CGTCGACACCGACTACGAC | GGTCAGCGGTGTACTCGAC |
| 4. Rf1 Troponin-1 [wup-like] (Trop-1) | CB518302 | CGACCTAGAATACGAAGTGG | TTCTTCTCCTTGTCCTCCTCC |
| 5. Rf2 Troponin-1 [wap-like] (Trop-2) | CB518303 | GAAGAGTTGAAGAAGGAGCAGG | TTGTTCACCAGGCTATTCAGG |
| Transcription and translation factors | |||
| 6. nanos | BQ788190 | CCACTGACTAAATGTATGGG | TTCAAGCCTCAACACTCTGT |
| 7. COP9 Complex Homolog Subunit 5 (COP9) | DN792518 | CTCGATCAGGAGGCACACTC | TTGCTGCCTCAATGTATGCT |
| 8. BTB-POZ [broad-like] | AY258590 | CTGGACCAGCATCTACATCTTC | GGTTGAAGCCTGATTCACAAG |
| 9. 18S rRNA-like (18S) | AY572860 | TATCGATCCTTTTGGCTTGG | TCGCAATGATAGGAAGAGCC |
| 10. 28S rRNA-like (28S) | CK906357 | GCGAATGATTAGAGGCCTTG | ACAGCGCCAGTTCTGCTTAC |
| 11. bicaudal (bic) | AY258589 | GAGGCAAGTACGGATTGGAG | CTCTTCATGGTAACAACCAG |
| 12. LIM | CB518301 | GTGCTTCAAGTGTGGCATGT | GTCCATGCTGAGACAACCAG |
| Signal transduction factors | |||
| 13. Malonyl Co-A Decarboxylase (M-CoA) | AY572861 | GCTACCGGCGACTCTTAATC | GAGGACACGCTGATTCCTTC |
| 14. Phospho-Inositol Phosphate (PIP) Kinase | CK906365 | AATTCTGCTGCCTTCTCTGG | ACCTTGCTTGGTCAACCATC |
| 15. Apoptosis Inhibitor (Apop) | CK906364 | CGTACATGTGTGAGCAGGTG | ATCACCATCAGGTGGCAGAG |
| 16. AMP Deaminase (AMP-de) | CK906363 | GTGACATGTGTGAGCTTGCC | ACCAGCTACACCTTCCTTGG |
| 17. GTPase Activating Protein (GAP) | BQ788178 | TTCCAACAGCACAAGAGCAC | TAACTGGTTGCGACAGGCAC |
| 18. ATPase | BQ788171 | TCAGGAAGTCTTGGATTCGG | TACGAACTCTGGTGCGTCTG |
| 19. SH3 Domain Kinase (SH3) | CB518513 | CGTGTTGCCAATGAGTTGAG | ACAATCCTATTCGGCCATCC |
| Reference/control genes | |||
| 20. β-actin | DQ206832 | AGAGGGAAATCGTGCGTGAC | CAATAGTGATGACCTGGCCGT |
| 21. NADH-dh | BQ788175 | GCTGGGGGTGTTATTCATTCCTA | GGCATACCACAAAGAGCAAAA |
| 22. HSP-70 | BQ788164 | AGAACCAAGTGGCCATGAAC | CCAATGCTTCATGTCTGCC |