Antibodies used in immunostaining analyses
| Antibody . | Species . | Dilution . | Company . |
|---|---|---|---|
| Anti-p53(clone pAb421) | Rabbit | 1:500 | Oncogene Research Products |
| Anti-Scp3 | Rabbit | 1:100 | Novus Biologicals |
| Anti-phospho-H2A.X (Ser139) | Rabbit | 1:500 | Upstate |
| Anti-ubiquityl-Histone H2A(clone E6C5) | Mouse | 1:100 | Upstate |
| Anti-monomethyl-Histone H3(Lys9) | Rabbit | 1:100 | Upstate |
| Anti-dimethyl-Histone H3(Lys9) | Rabbit | 1:100 | Upstate |
| Anti-acetyl-Histone H3 | Rabbit | 1:100 | Upstate |
| Anti-trimethyl-Histone H3 (Lys27) | Rabbit | 1:100 | Upstate |
| Anti-dimethyl-Histone H4 (Lys20) | Rabbit | 1:100 | Upstate |
| Anti-monomethyl-Histone H3 (Lys4) | Rabbit | 1:100 | Upstate |
| Anti-Rad51 (H-92) | Rabbit | 1:50 | Santa Cruz |
| Anti-Mlh1 (G168-15) | Mouse | 1:50 | BD Pharmingen |
| Anti-phosphorylated RNA polymerase II | Mouse | 1:25 | Covance |
| Anti-Scmh1 | Mouse | Undiluted | This study |
| Anti-Phc1 | Mouse | Undiluted | Miyagishima et al., 2003 |
| Anti-Phc2 | Mouse | Undiluted | Isono et al., 2005 |
| Anti-Bmi1(H-99) | Rabbit | 1:25 | Santa Cruz |
| Anti-Rnf1 10(C-20) | Rabbit | 1:30 | Santa Cruz |
| Anti-Cbx2(C-18) | Rabbit | 1:25 | Santa Cruz |
| Anti-Rnf2 | Mouse | Undiluted | Atsuta et al., 2001 |
| Anti-Ezh2 | Rabbit | 1:100 | Upstate |
| Anti-mouse IgM FITC | Donkey | 1:100 | Becton Dickinson |
| Anti-mouse IgG Cy2 | Donkey | 1:100 | Jackson ImmunoResearch Laboratories |
| Anti-rabbit IgG Cy3 | Donkey | 1:500 | Jackson ImmunoResearch Laboratories |
| Anti-mouse IgG (H+L) Alexa Fluor 488 | Goat | 1:300 | Molecular Probes |
| Anti-rabbit IgG (H+L) Alexa Fluor 568 | Goat | 1:300 | Molecular Probes |
| Anti-rabbit IgG, HRP-conjugated | Goat | 1:2000 | Amersham |
| Antibody . | Species . | Dilution . | Company . |
|---|---|---|---|
| Anti-p53(clone pAb421) | Rabbit | 1:500 | Oncogene Research Products |
| Anti-Scp3 | Rabbit | 1:100 | Novus Biologicals |
| Anti-phospho-H2A.X (Ser139) | Rabbit | 1:500 | Upstate |
| Anti-ubiquityl-Histone H2A(clone E6C5) | Mouse | 1:100 | Upstate |
| Anti-monomethyl-Histone H3(Lys9) | Rabbit | 1:100 | Upstate |
| Anti-dimethyl-Histone H3(Lys9) | Rabbit | 1:100 | Upstate |
| Anti-acetyl-Histone H3 | Rabbit | 1:100 | Upstate |
| Anti-trimethyl-Histone H3 (Lys27) | Rabbit | 1:100 | Upstate |
| Anti-dimethyl-Histone H4 (Lys20) | Rabbit | 1:100 | Upstate |
| Anti-monomethyl-Histone H3 (Lys4) | Rabbit | 1:100 | Upstate |
| Anti-Rad51 (H-92) | Rabbit | 1:50 | Santa Cruz |
| Anti-Mlh1 (G168-15) | Mouse | 1:50 | BD Pharmingen |
| Anti-phosphorylated RNA polymerase II | Mouse | 1:25 | Covance |
| Anti-Scmh1 | Mouse | Undiluted | This study |
| Anti-Phc1 | Mouse | Undiluted | Miyagishima et al., 2003 |
| Anti-Phc2 | Mouse | Undiluted | Isono et al., 2005 |
| Anti-Bmi1(H-99) | Rabbit | 1:25 | Santa Cruz |
| Anti-Rnf1 10(C-20) | Rabbit | 1:30 | Santa Cruz |
| Anti-Cbx2(C-18) | Rabbit | 1:25 | Santa Cruz |
| Anti-Rnf2 | Mouse | Undiluted | Atsuta et al., 2001 |
| Anti-Ezh2 | Rabbit | 1:100 | Upstate |
| Anti-mouse IgM FITC | Donkey | 1:100 | Becton Dickinson |
| Anti-mouse IgG Cy2 | Donkey | 1:100 | Jackson ImmunoResearch Laboratories |
| Anti-rabbit IgG Cy3 | Donkey | 1:500 | Jackson ImmunoResearch Laboratories |
| Anti-mouse IgG (H+L) Alexa Fluor 488 | Goat | 1:300 | Molecular Probes |
| Anti-rabbit IgG (H+L) Alexa Fluor 568 | Goat | 1:300 | Molecular Probes |
| Anti-rabbit IgG, HRP-conjugated | Goat | 1:2000 | Amersham |