Primers used in semiquantitative RT-PCR analyses
| Gene . | Forward (5′→3′) . | Reverse (5′→3′) . |
|---|---|---|
| A-myb | aagaagttggttgaacaacacgg | aggaagtaacttagcaatctcgg |
| Dmc1 | ttcgtactggaaaaactcagctgtatc | cttggctgcgacataatcaagtagctcc |
| Mvh1 | ccaaaagtgacatatataccc | ttggttgatcacttctcgag |
| Scp-3 | ggtggaagaaagcattctgg | cagctccaaatttttccagc |
| CyclinA1 | atgcatcgccagagctccaagag | ggaagtggagatctgacttgagc |
| Calmegin | atatgcgtttccagggtgttggac | gtatgcacctccacaatcaatacc |
| Bmp8a | ggctcgagatggtggtcaaggcctgtgg | ggggatccaggctctttctatgtggcc |
| CREMτ | gattgaagaagaaaaatcaga | catgctgtaatcagttcatag |
| β-actin | gagagggaaatcgtgcgtga | acatctgctggaaggtggac |
| Scmh1 | ||
| Primers 1/2 | atgctggtttgctac | aggacaaaggtttcacct |
| Primers 3/4 | actgccacagagtataatca | tcagaacttgccctg |
| Gene . | Forward (5′→3′) . | Reverse (5′→3′) . |
|---|---|---|
| A-myb | aagaagttggttgaacaacacgg | aggaagtaacttagcaatctcgg |
| Dmc1 | ttcgtactggaaaaactcagctgtatc | cttggctgcgacataatcaagtagctcc |
| Mvh1 | ccaaaagtgacatatataccc | ttggttgatcacttctcgag |
| Scp-3 | ggtggaagaaagcattctgg | cagctccaaatttttccagc |
| CyclinA1 | atgcatcgccagagctccaagag | ggaagtggagatctgacttgagc |
| Calmegin | atatgcgtttccagggtgttggac | gtatgcacctccacaatcaatacc |
| Bmp8a | ggctcgagatggtggtcaaggcctgtgg | ggggatccaggctctttctatgtggcc |
| CREMτ | gattgaagaagaaaaatcaga | catgctgtaatcagttcatag |
| β-actin | gagagggaaatcgtgcgtga | acatctgctggaaggtggac |
| Scmh1 | ||
| Primers 1/2 | atgctggtttgctac | aggacaaaggtttcacct |
| Primers 3/4 | actgccacagagtataatca | tcagaacttgccctg |