The phenotypes of hai1a mutants, hai1a morphants, and embryos lacking both Hai1a and Hai1b activity are rescued upon (co-)injection of matriptase1a MO
| . | . | Phenotype (n) . | . | . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| hai1a genotype of parents . | MO injection . | WT . | hai1 normal . | hai1 strong . | Lysed . | n (total) . | % WT . | # exps . | |||
| +/− | - | 63 | 20 | 0 | 0 | 83 | 75.9 | 2 | |||
| +/− | matriptase1a | 252 | 3 | 0 | 0 | 255 | 98.8 | 2 | |||
| −/− | - | 0 | 72 | 0 | 0 | 72 | 0 | 2 | |||
| −/− | matriptase1a | 153 | 0 | 0 | 0 | 153 | 100 | 2 | |||
| +/+ | hai1a | 8 | 66 | 0 | 0 | 74 | 10.8 | 1 | |||
| +/+ | hai1a + matriptase1a | 167 | 6 | 0 | 0 | 173 | 96.5 | 1 | |||
| −/− | hai1b | 0 | 0 | 63 | 41 | 104 | 0 | 2 | |||
| −/− | hai1a + matriptase1a | 56 | 48 | 45 | 22 | 171 | 32.7 | 2 | |||
| . | . | Phenotype (n) . | . | . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| hai1a genotype of parents . | MO injection . | WT . | hai1 normal . | hai1 strong . | Lysed . | n (total) . | % WT . | # exps . | |||
| +/− | - | 63 | 20 | 0 | 0 | 83 | 75.9 | 2 | |||
| +/− | matriptase1a | 252 | 3 | 0 | 0 | 255 | 98.8 | 2 | |||
| −/− | - | 0 | 72 | 0 | 0 | 72 | 0 | 2 | |||
| −/− | matriptase1a | 153 | 0 | 0 | 0 | 153 | 100 | 2 | |||
| +/+ | hai1a | 8 | 66 | 0 | 0 | 74 | 10.8 | 1 | |||
| +/+ | hai1a + matriptase1a | 167 | 6 | 0 | 0 | 173 | 96.5 | 1 | |||
| −/− | hai1b | 0 | 0 | 63 | 41 | 104 | 0 | 2 | |||
| −/− | hai1a + matriptase1a | 56 | 48 | 45 | 22 | 171 | 32.7 | 2 | |||
Compare results of table with those shown in Fig. 7. Phenotypes were evaluated at 24 hpf, based on skin morphology, n, number of embryos;% WT, percentage of embryos with wild-type phenotype; # exps, number of evaluated experiments.