kgb-1 is RNAi-sensitive; csn-5(RNAi) indicates that KGB-1 and CSN-5 interact
| dsRNA injected into: . | Number of worms injected . | Number with RNAi effect in all F1s . | Number F1s without RNAi effect . | % F1s with RNAi effect . |
|---|---|---|---|---|
| actin into N2 | 23 | 19 | 4* | 83 |
| actin into kgb-1 | 21 | 19 | 2* | 90 |
| pan-1 into N2 | 24 | 20† | 4‡ | 83 |
| pan-1 into kgb-1 | 26 | 24† | 2‡ | 92 |
| csn-5 into N2 | 63 | 47 | 16§ | 75 |
| csn-5 into kgb-1 | 63 | 23 | 40§ | 37 |
| dsRNA injected into: . | Number of worms injected . | Number with RNAi effect in all F1s . | Number F1s without RNAi effect . | % F1s with RNAi effect . |
|---|---|---|---|---|
| actin into N2 | 23 | 19 | 4* | 83 |
| actin into kgb-1 | 21 | 19 | 2* | 90 |
| pan-1 into N2 | 24 | 20† | 4‡ | 83 |
| pan-1 into kgb-1 | 26 | 24† | 2‡ | 92 |
| csn-5 into N2 | 63 | 47 | 16§ | 75 |
| csn-5 into kgb-1 | 63 | 23 | 40§ | 37 |
For RNAi, N2 or kgb-1 young adults at 20°C were injected,individually plated and purged of maternal protein. For actin and pan-1(RNAi), injected worms were scored for producing dead eggs(actin) or arrested L2-sized larvae (pan-1) 3 days after injection. Student's t-tests of differences between N2 and kgb-1 are highly significant for csn-5(P<1×10−5); with no significant differences between N2 and kgb-1 for actin or pan-1, indicating kgb-1(um3) is not RNAi defective.
After csn-5(RNAi), F1s were scored on day 5-6 as producing no F2 or §producing one (or more)F2s.
Injected nematodes designated as actin `escapers' had 1-2 live progeny that grew to fertile adults.
One pan-1-injected N2 and one pan-1-injected kgb-1 became sterile adults and were included with those showing RNAi effects.
pan-1 `escapers' had 1-2 larvae that became fertile adults.