GLH-1, CSN-5 and Vasa contain consensus motifs used by MAP kinases
| . | MAPK docking (D) sites . | Phosphodegron site . |
|---|---|---|
| Consensus | K/R-X-X/K/R-K/R-X(1-4)-L/I-X-L/I or K/R-K/R-K/R-X(1-5)-L/I-X-L/I | I/L-I/L/P-pT-P-notK/R |
| GLH-1 (aa 581) | KKDKLLEL consensus | GLH-1 (aa 423) ILTPT consensus |
| GLH-4 (aa 977) | KRDAIYKLLGI | GLH-4 (aa 820) IVAPT |
| GLH-4 (aa 977) | KRDAIYKLLGI | GLH-4 (aa 758) RPTPI |
| CSN-5 (aa 54) | KQIKISAIAL consensus | Vasa (aa 267) IPTPI consensus |
| Vasa (aa 475) | KRSKLIEI consensus |
| . | MAPK docking (D) sites . | Phosphodegron site . |
|---|---|---|
| Consensus | K/R-X-X/K/R-K/R-X(1-4)-L/I-X-L/I or K/R-K/R-K/R-X(1-5)-L/I-X-L/I | I/L-I/L/P-pT-P-notK/R |
| GLH-1 (aa 581) | KKDKLLEL consensus | GLH-1 (aa 423) ILTPT consensus |
| GLH-4 (aa 977) | KRDAIYKLLGI | GLH-4 (aa 820) IVAPT |
| GLH-4 (aa 977) | KRDAIYKLLGI | GLH-4 (aa 758) RPTPI |
| CSN-5 (aa 54) | KQIKISAIAL consensus | Vasa (aa 267) IPTPI consensus |
| Vasa (aa 475) | KRSKLIEI consensus |
Amino acids matching putative consensus motifs are underlined; those in bold are non-consensus and those unmarked are amino acids not discriminatory for the MAPK D motif. Variations of the D site consensus have been reported,with GLH-1 matching one, and GLH-4 having one mismatch for either of two shown. CSN-5 has a consensus D site, as does Vasa. Both GLH-1 and Vasa have consensus phosphodegrons; although GLH-4 has two potential sites, neither is consensus. PGL-1 has no D site, nor a phosphodegron (not shown). Sequences were aligned using the NCBI BLAST program (bl2seq blastp).