Quantitative analysis of growth, embryo turning and yolk sac vessel remodeling in control and treated embryos
| Treatment . | n . | Yolk sac (mm2) . | Turn score . | Remodel score . |
|---|---|---|---|---|
| Untreated control | 23 | 5.23±0.19 | 3.78±0.32 | 4.13±0.30 |
| Low hematocrit | 41 | 4.06±0.12* | 1.70±0.15* | 1.76±0.16* |
| Low hematocrit+hetastarch | 19 | 5.19±0.28 | 2.68±0.32 | 3.58±0.30 |
| Acrylamide only | 5 | 4.93±0.41 | 3.80±0.49 | 4.20±0.49 |
| TEMED only | 14 | 5.93±0.28 | 4.00±0.36 | 4.64±0.20 |
| Hetastarch only | 12 | 6.05±0.24 | 3.50±0.44 | 4.25±0.37 |
| Treatment . | n . | Yolk sac (mm2) . | Turn score . | Remodel score . |
|---|---|---|---|---|
| Untreated control | 23 | 5.23±0.19 | 3.78±0.32 | 4.13±0.30 |
| Low hematocrit | 41 | 4.06±0.12* | 1.70±0.15* | 1.76±0.16* |
| Low hematocrit+hetastarch | 19 | 5.19±0.28 | 2.68±0.32 | 3.58±0.30 |
| Acrylamide only | 5 | 4.93±0.41 | 3.80±0.49 | 4.20±0.49 |
| TEMED only | 14 | 5.93±0.28 | 4.00±0.36 | 4.64±0.20 |
| Hetastarch only | 12 | 6.05±0.24 | 3.50±0.44 | 4.25±0.37 |
After 24 hours in roller culture, embryos were individually imaged. The size of the yolk sac is the area of the traced image, and the embryo was assigned a score for degree of turning and degree of remodeling (see Materials and methods). A score of 1 describes an unturned embryo or unremodeled yolk sac, and a score of 5 describes a normally turned embryo and normally remodeled yolk sac.
Significant difference (P<0.05) from control value.