Skip to Main Content
Table 2.

In vivo LTR (primary transplantation) and HSC activity (secondary transplantation) of Mpl−/− and C57Bl6 FL,placenta and yolk sac

Primary transplantation (LTR)
Secondary transplantation (HSC)
No. organs or cells injectedEngrafted mice/Total miceMedian of reconstitution (%)No. cells injectedEngrafted mice/Total miceMedian of reconstitution (%)
Fetal liver E11.5 Mpl−/− 0/5   ND ND 
   0/8     
   0/6     
   4
 
0/3
 

 

 
  
  C57Bl6 0.5 1/10 91  ND ND 
   1
 
7/10
 
72
 

 
  
 E12.5 Mpl−/− 5×104 10/15 90 5×105 4/10 11 
   1×105
 
11/15
 
89
 
   
  C57Bl6 5×104 2/15 78 5×105 4/10 35 
   1×105
 
7/15
 
83
 
   
 E14.5 Mpl−/− 1×104 0/5  5×105 4/10 25 
   5×104
 
2/5
 
91
 
   
  C57Bl6 1×104 1/5 81 5×105 9/10 51 
   5×104 3/5 80    
Placenta E12.5 Mpl−/− 0×1 3/15 79 5×105 5/15 21 
   0×3
 
10/20
 
70
 
   
  C57Bl6 0×1 2/15 49 5×105 10/10 49 
   0×3 8/20 88    
YS E12.5 Mpl−/− 3/12 85  ND ND 
  C57Bl6 0/10     
Primary transplantation (LTR)
Secondary transplantation (HSC)
No. organs or cells injectedEngrafted mice/Total miceMedian of reconstitution (%)No. cells injectedEngrafted mice/Total miceMedian of reconstitution (%)
Fetal liver E11.5 Mpl−/− 0/5   ND ND 
   0/8     
   0/6     
   4
 
0/3
 

 

 
  
  C57Bl6 0.5 1/10 91  ND ND 
   1
 
7/10
 
72
 

 
  
 E12.5 Mpl−/− 5×104 10/15 90 5×105 4/10 11 
   1×105
 
11/15
 
89
 
   
  C57Bl6 5×104 2/15 78 5×105 4/10 35 
   1×105
 
7/15
 
83
 
   
 E14.5 Mpl−/− 1×104 0/5  5×105 4/10 25 
   5×104
 
2/5
 
91
 
   
  C57Bl6 1×104 1/5 81 5×105 9/10 51 
   5×104 3/5 80    
Placenta E12.5 Mpl−/− 0×1 3/15 79 5×105 5/15 21 
   0×3
 
10/20
 
70
 
   
  C57Bl6 0×1 2/15 49 5×105 10/10 49 
   0×3 8/20 88    
YS E12.5 Mpl−/− 3/12 85  ND ND 
  C57Bl6 0/10     

Single-cell suspensions of FL, placenta and yolk sac were injected into irradiated adult recipients. For LTR activity, each res ult represents the number of engrafted mice at ≥20 weeks post-transplantation out of the total number of mice transplanted. Two or three independent experiments were performed. For secondary transplantations, 5×105 bone marrow cells isolated from primary recipients with high chimerism were injected, and mice were analyzed 7 weeks post-transplantation (two independent experiments).

ND, not determined.

Close Modal

or Create an Account

Close Modal
Close Modal