In vivo LTR (primary transplantation) and HSC activity (secondary transplantation) of Mpl−/− and C57B16 AGM
| . | . | . | Primary transplantation (LTR) . | . | . | Secondary transplantation (HSC) . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | No. organs or cells injected . | Engrafted mice/Total mice . | Median of reconstitution (%) . | No. cells injected . | Engrafted mice/Total mice . | Median of reconstitution (%) . | ||||
| AGM | E11.5 | Mpl−/− | 1 | 1/7 | 16 | 5×105 | 6/10 | 28 | ||||
| 3 | 2/6 | 75 | ||||||||||
| C57B16 | 1 | 3/10 | 78 | 5×105 | 7/10 | 63 | ||||||
| 3 | 4/7 | 89 | ||||||||||
| E12.5 | Mpl−/− | 1 | 6/10 | 62 | 5×105 | 6/10 | 6 | |||||
| C57B16 | 1 | 1/10 | 81 | 5×105 | 8/10 | 38 | ||||||
| . | . | . | Primary transplantation (LTR) . | . | . | Secondary transplantation (HSC) . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | No. organs or cells injected . | Engrafted mice/Total mice . | Median of reconstitution (%) . | No. cells injected . | Engrafted mice/Total mice . | Median of reconstitution (%) . | ||||
| AGM | E11.5 | Mpl−/− | 1 | 1/7 | 16 | 5×105 | 6/10 | 28 | ||||
| 3 | 2/6 | 75 | ||||||||||
| C57B16 | 1 | 3/10 | 78 | 5×105 | 7/10 | 63 | ||||||
| 3 | 4/7 | 89 | ||||||||||
| E12.5 | Mpl−/− | 1 | 6/10 | 62 | 5×105 | 6/10 | 6 | |||||
| C57B16 | 1 | 1/10 | 81 | 5×105 | 8/10 | 38 | ||||||
Single-cell suspensions of AGM were injected into irradiated adult recipients. For LTR activity, each result represents the num ber of engrafted mice at ≥20 weeks post-transplantation (showing more than 5% donor CD45.2 cells in peripheral blood) out of the total number of mice transplanted. Two or three independent experiments were performed. For secondary transplantations, 5×105 bone marrow cells isolated from primary recipients with high chimerism were injected, and mice were analyzed 7 weeks post-transplantation (two independent experiments).