Skip to Main Content
Table 1.

In vivo LTR (primary transplantation) and HSC activity (secondary transplantation) of Mpl−/− and C57B16 AGM

Primary transplantation (LTR)
Secondary transplantation (HSC)
No. organs or cells injectedEngrafted mice/Total miceMedian of reconstitution (%)No. cells injectedEngrafted mice/Total miceMedian of reconstitution (%)
AGM E11.5 Mpl−/− 1/7 16 5×105 6/10 28 
   3
 
2/6
 
75
 
   
  C57B16 3/10 78 5×105 7/10 63 
   3
 
4/7
 
89
 
   
 E12.5 Mpl−/− 6/10 62 5×105 6/10 
  C57B16 1/10 81 5×105 8/10 38 
Primary transplantation (LTR)
Secondary transplantation (HSC)
No. organs or cells injectedEngrafted mice/Total miceMedian of reconstitution (%)No. cells injectedEngrafted mice/Total miceMedian of reconstitution (%)
AGM E11.5 Mpl−/− 1/7 16 5×105 6/10 28 
   3
 
2/6
 
75
 
   
  C57B16 3/10 78 5×105 7/10 63 
   3
 
4/7
 
89
 
   
 E12.5 Mpl−/− 6/10 62 5×105 6/10 
  C57B16 1/10 81 5×105 8/10 38 

Single-cell suspensions of AGM were injected into irradiated adult recipients. For LTR activity, each result represents the num ber of engrafted mice at ≥20 weeks post-transplantation (showing more than 5% donor CD45.2 cells in peripheral blood) out of the total number of mice transplanted. Two or three independent experiments were performed. For secondary transplantations, 5×105 bone marrow cells isolated from primary recipients with high chimerism were injected, and mice were analyzed 7 weeks post-transplantation (two independent experiments).

Close Modal

or Create an Account

Close Modal
Close Modal