The Company of Biologists

Table 2.

Summary of the embryonic phenotypes in the Egfl7-knockout line^{^*}

		*Phenotypic category in the Egfl7^−/− embryos% (n)*							*Phenotypic category in the Egfl7^+/+ and Egfl7^+/− embryos % (n)*
Age	Total number of embryos analyzed	No observed phenotype	Systemic oedema	No heart beat and systemic oedema	Number of Egfl7^−/− embryos	No observed phenotype	Systemic oedema	No heart beat	Number of Egfl7^+/+ and Egfl7^+/−embryos
E9.5-12.5	109	100 (19)	0 (0)	0 (0)	19	98.9 (89)	0 (0)	1.1 (1^{^†})	90
E13.5	35	44.4 (4)	55.6 (5)	0 (0)	9	96.2 (25)	0 (0)	3.8 (1^{^‡})	26
E14.5	93	45.5 (15)	39.3 (13)	15.2 (5)	33	100 (60)	0 (0)	0 (0)	60
E15.5	21	33.3 (1)	0 (0)	66.7 (2)	3	100 (18)	0 (0)	0 (0)	18

		*Phenotypic category in the Egfl7^−/− embryos% (n)*							*Phenotypic category in the Egfl7^+/+ and Egfl7^+/− embryos % (n)*
Age	Total number of embryos analyzed	No observed phenotype	Systemic oedema	No heart beat and systemic oedema	Number of Egfl7^−/− embryos	No observed phenotype	Systemic oedema	No heart beat	Number of Egfl7^+/+ and Egfl7^+/−embryos
E9.5-12.5	109	100 (19)	0 (0)	0 (0)	19	98.9 (89)	0 (0)	1.1 (1^{^†})	90
E13.5	35	44.4 (4)	55.6 (5)	0 (0)	9	96.2 (25)	0 (0)	3.8 (1^{^‡})	26
E14.5	93	45.5 (15)	39.3 (13)	15.2 (5)	33	100 (60)	0 (0)	0 (0)	60
E15.5	21	33.3 (1)	0 (0)	66.7 (2)	3	100 (18)	0 (0)	0 (0)	18

Values in bold are statistically significant.

Insertional knockout in the 129svj/C57Bl/6 mixed background.

This is an Egfl7^+/+ E12.5 embryo. It did not have oedema.

This is an Egfl7^+/− embryo. It did not have oedema.