Mendelian ratios of the Egfl7-knockout lines
| . | . | . | Ratios for each genotype . | . | . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | Observed % (n) . | . | . | Expected % (n) . | . | . | . | . | |||||
| . | Genetic background (generation) . | . | Genotype . | . | . | Genotype . | . | . | Total number of animals analyzed (n) . | P values of observed vs expected (−/−) ratios(ϰ2 test) . | |||||
| Knockout line . | . | Age . | +/+ . | +/− . | −/− . | +/+ . | +/− . | −/− . | . | . | |||||
| Insertional knockout | 129svj/C57Bl/6 mixed | Adult | 33.1 (115) | 53.3 (185) | 13.5 (47) | 25 (87) | 50 (173) | 25 (87) | 347 | <0.0001* | |||||
| 129svj/C57Bl/6 mixed | P0-8 | 33.2 (68) | 53.7 (110) | 13.1 (27) | 25 (51) | 50 (103) | 25 (51) | 205 | <0.0001* | ||||||
| 129svj/C57Bl/6 mixed | E9.5-15.5 | 24 (62) | 51.2 (132) | 24.8 (64) | 25 (64.5) | 50 (129) | 25 (64.5) | 258 | 0.9185 | ||||||
| Homologous recombination knockout | C57Bl/6 (N5) | Adult | 33.9 (96) | 52 (147) | 14.1 (40) | 25 (71) | 50 (141) | 25 (71) | 283 | <0.0001* | |||||
| C57Bl/6 (N10) | Adult | 31.5 (73) | 59 (137) | 9.5 (22) | 25 (58) | 50 (116) | 25 (58) | 232 | <0.0001* (0.0921 N10 vs N5) | ||||||
| 129svj Pure | Adult | 36.6 (30) | 48.8 (40) | 14.6 (12) | 25 (20.5) | 50 (41) | 25 (20.5) | 82 | 0.0188* | ||||||
| C57Bl/6 (N5) | P0-8 | 31.7 (91) | 54.7 (157) | 13.6 (39) | 25 (72) | 50 (143) | 25 (72) | 287 | <0.0001* | ||||||
| C57Bl/6 (N0-5) | E9.5-15.5 | 28.5 (84) | 46.1 (136) | 25.4 (75) | 25 (74) | 50 (147) | 25 (74) | 295 | 0.3104 | ||||||
| . | . | . | Ratios for each genotype . | . | . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | Observed % (n) . | . | . | Expected % (n) . | . | . | . | . | |||||
| . | Genetic background (generation) . | . | Genotype . | . | . | Genotype . | . | . | Total number of animals analyzed (n) . | P values of observed vs expected (−/−) ratios(ϰ2 test) . | |||||
| Knockout line . | . | Age . | +/+ . | +/− . | −/− . | +/+ . | +/− . | −/− . | . | . | |||||
| Insertional knockout | 129svj/C57Bl/6 mixed | Adult | 33.1 (115) | 53.3 (185) | 13.5 (47) | 25 (87) | 50 (173) | 25 (87) | 347 | <0.0001* | |||||
| 129svj/C57Bl/6 mixed | P0-8 | 33.2 (68) | 53.7 (110) | 13.1 (27) | 25 (51) | 50 (103) | 25 (51) | 205 | <0.0001* | ||||||
| 129svj/C57Bl/6 mixed | E9.5-15.5 | 24 (62) | 51.2 (132) | 24.8 (64) | 25 (64.5) | 50 (129) | 25 (64.5) | 258 | 0.9185 | ||||||
| Homologous recombination knockout | C57Bl/6 (N5) | Adult | 33.9 (96) | 52 (147) | 14.1 (40) | 25 (71) | 50 (141) | 25 (71) | 283 | <0.0001* | |||||
| C57Bl/6 (N10) | Adult | 31.5 (73) | 59 (137) | 9.5 (22) | 25 (58) | 50 (116) | 25 (58) | 232 | <0.0001* (0.0921 N10 vs N5) | ||||||
| 129svj Pure | Adult | 36.6 (30) | 48.8 (40) | 14.6 (12) | 25 (20.5) | 50 (41) | 25 (20.5) | 82 | 0.0188* | ||||||
| C57Bl/6 (N5) | P0-8 | 31.7 (91) | 54.7 (157) | 13.6 (39) | 25 (72) | 50 (143) | 25 (72) | 287 | <0.0001* | ||||||
| C57Bl/6 (N0-5) | E9.5-15.5 | 28.5 (84) | 46.1 (136) | 25.4 (75) | 25 (74) | 50 (147) | 25 (74) | 295 | 0.3104 | ||||||
The offspring of multiple pairs of Egfl7+/−parents in different genetic background were genotyped and counted. The N values refer to how many generations the mutant mice in the 129svj/C57Bl/6 mixed background have been crossed to C57Bl/6 mice. Values in bold are statistically significant (*).