Defects in POP-1 and GFP::SYS-1 asymmetry in various genetic backgrounds
| . | MSa/MSp . | . | Ea/Ep . | . | AB8 . | . | AB16 . | . | AB32 . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | SYS-1* . | POP-1 . | SYS-1 . | POP-1 . | SYS-1 . | POP-1 . | SYS-1 . | POP-1 . | SYS-1 . | POP-1 . | |||||
| Wild-type N2 | 1 (132)† | 5 (131) | 1 (79) | 9 (75) | 0 (7) | 0 (7) | 0 (18) | 0 (18) | 0 (6) | 0 (6) | |||||
| mom-2(or42) | 100 (26) | 100‡(26) | 79 (33) | 76 (33) | 100 (5) | 40 (5) | 17 (12) | 0 (12) | 0 (2) | 0 (2) | |||||
| mom-5(or57) | 100 (6) | 86 (7) | 100 (8) | 87 (8) | 100 (6) | 88 (6) | 65 (17) | 59 (17) | 11 (9) | 11 (9) | |||||
| apr-1(RNAi) | 100 (15) | 100 (14) | 93 (14) | 87 (15) | |||||||||||
| lit-1(t1512) | 0 (19) | 100 (19) | 22 (9) | 100 (9) | |||||||||||
| mom-4(ne19) | 0 (16) | 100 (16) | 0 (19) | 100 (19) | |||||||||||
| wrm-1(RNAi) | 0 (37) | 100 (37) | 0 (20) | 100 (20) | 0 (6) | 100 (6) | 0 (10) | 100 (10) | 0 (3) | 100 (3) | |||||
| wrm-1(ne1982) | 11 (19)§ | 53 (19) | 0 (22) | 32 (22) | |||||||||||
| . | MSa/MSp . | . | Ea/Ep . | . | AB8 . | . | AB16 . | . | AB32 . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | SYS-1* . | POP-1 . | SYS-1 . | POP-1 . | SYS-1 . | POP-1 . | SYS-1 . | POP-1 . | SYS-1 . | POP-1 . | |||||
| Wild-type N2 | 1 (132)† | 5 (131) | 1 (79) | 9 (75) | 0 (7) | 0 (7) | 0 (18) | 0 (18) | 0 (6) | 0 (6) | |||||
| mom-2(or42) | 100 (26) | 100‡(26) | 79 (33) | 76 (33) | 100 (5) | 40 (5) | 17 (12) | 0 (12) | 0 (2) | 0 (2) | |||||
| mom-5(or57) | 100 (6) | 86 (7) | 100 (8) | 87 (8) | 100 (6) | 88 (6) | 65 (17) | 59 (17) | 11 (9) | 11 (9) | |||||
| apr-1(RNAi) | 100 (15) | 100 (14) | 93 (14) | 87 (15) | |||||||||||
| lit-1(t1512) | 0 (19) | 100 (19) | 22 (9) | 100 (9) | |||||||||||
| mom-4(ne19) | 0 (16) | 100 (16) | 0 (19) | 100 (19) | |||||||||||
| wrm-1(RNAi) | 0 (37) | 100 (37) | 0 (20) | 100 (20) | 0 (6) | 100 (6) | 0 (10) | 100 (10) | 0 (3) | 100 (3) | |||||
| wrm-1(ne1982) | 11 (19)§ | 53 (19) | 0 (22) | 32 (22) | |||||||||||
SYS-1 was scored by immunofluorescence using the anti-GFP antibody in teEx321 and tels98 embryos.
Numbers indicate percentage of sister pairs scored with abnormal asymmetry. Numbers in parenthesis are total number of pairs scored. Abnormal asymmetry is defined as a pattern different from wild type. Sister pairs with only subtle differences were scored as abnormal. For AB8, AB16 and AB32, abnormality was scored if some or all pairs of AB cells at that particular stage were scored as abnormal.
Most pairs of sister cells scored as having abnormal POP-1 asymmetry in mom-2, mom-5 and apr-1 embryos, still had subtle POP-1 asymmetry.
We observed abnormal SYS-1 asymmetry between the MSa/MSp pair in two ne1982 embryos. One embryo had only a subtle difference between MSa and MSp, whereas the other had a reversed asymmetry.