Penetrance of gutless phenotype in various mutants with altered SYS-1 or POP-1 levels
| . | Reported . | This study . |
|---|---|---|
| skn-1(zu67) | 67* | 63 (46)† |
| sys-1 (RNAi) | 4 (429) | |
| skn-1(zu67); sys-1 (RNAi) | 100 (68) | |
| skn-1(RNAi)(inj)‡ | 74 (46) | |
| skn-1(RNAi) | 72 (163) | |
| skn-1(RNAi)(inj); mom-2 (or42) | 100§ | 100 (71) |
| skn-1(RNAi)(inj); mom-4 (ne19) | 100 (79) | |
| skn-1(RNAi); mom-5(or57) | 96 (179) | |
| teEx321(Pmed-1gfp::sys-1)¶ | 0 (134) | |
| tels3(Pmed-1gfp::pop-1)** | 0 (150) | |
| tels98(Ppie-1gfp::sys-1)** | 0 (392) | |
| skn-1(RNAi); tels98 | 16 (215) | |
| mom-1(or10) | 85§ | 83 (55) |
| mom-1(or10); sys-1(RNAi) | 93 (41) | |
| mom-1(or10); tels3 | 93 (41) | |
| mom-2(or42) | 73§ | 72 (105) |
| mom-2(RNAi) | 14‡‡ | 28 (49) |
| mom-2(or42); sys-1(RNAi) | 100 (229) | |
| mom-2(or42); teEx321 | 10 (39) | |
| mom-2(RNAi)††; tels3 | 60 (67) | |
| mom-2(or42); tels98 | 28 (145) | |
| mom-2(or42); tels98; skn-1(RNAi) | 94 (467) | |
| mom-3(or78) | 65§ | 70 (60) |
| mom-3(or78); sys-1(RNAi) | 81 (80) | |
| mom-3(or78); teEx321 | 13 (15) | |
| mom-3(or78); tels3 | 84 (50) | |
| mom-4(ne19) | 43‡‡ | 40 (65) |
| mom-4(ne19); sys-1(RNAi) | 100 (246) | |
| mom-4(ne19); teEx321 | 1 (67) | |
| mom-4(ne19); tels3 | 75 (56) | |
| mom-5(or57) | 5§ | 14 (113) |
| mom-5(or57); sys-1(RNAi) | 60 (134) | |
| mom-5(or57); teEx321 | 0 (62) | |
| mom-5(or57); tels3 | 65 (93) | |
| mom-5(or57); tels98 | 4 (131) | |
| mom-5(or57); tels98; skn-1(RNAi) | 52 (188) | |
| apr-1(RNAi) | 26‡‡ | 29 (70) |
| apr-1(RNAi); teEx321 | 0 (20) | |
| apr-1(RNAi); tels3 | 71 (62) | |
| lit-1(t1534) | 0§§ | 0 (116) |
| lit-1(t1534); sys-1(RNAi) | 100 (126) | |
| lit-1(t1534); tels3 | 94 (56) | |
| lit-1(1512) | 100§§ | 99 (224) |
| lit-1(t1512); teEx321 | 65 (31) | |
| wrm-1(RNAi) | 100‡‡ | 100 (122) |
| wrm-1(RNAi); sys-1(RNAi) | 100 (87) | |
| wrm-1(RNAi); teEx321 | 100 (143) | |
| wrm-1(RNAi); tels3 | 100 (170) | |
| med-1/2(RNAi)¶¶ | 7-52 | 16 (61) |
| med-1/2(RNAi); mom-2(or42) | 85 (66) | |
| med-1/2(RNAi); mom-4(ne19) | 46 (57) | |
| med-1/2(RNAi); mom-2(or42); teEx321 | 23 (31) | |
| med-1/2(RNAi); mom-4(ne19); teEx321 | 19 (27) |
| . | Reported . | This study . |
|---|---|---|
| skn-1(zu67) | 67* | 63 (46)† |
| sys-1 (RNAi) | 4 (429) | |
| skn-1(zu67); sys-1 (RNAi) | 100 (68) | |
| skn-1(RNAi)(inj)‡ | 74 (46) | |
| skn-1(RNAi) | 72 (163) | |
| skn-1(RNAi)(inj); mom-2 (or42) | 100§ | 100 (71) |
| skn-1(RNAi)(inj); mom-4 (ne19) | 100 (79) | |
| skn-1(RNAi); mom-5(or57) | 96 (179) | |
| teEx321(Pmed-1gfp::sys-1)¶ | 0 (134) | |
| tels3(Pmed-1gfp::pop-1)** | 0 (150) | |
| tels98(Ppie-1gfp::sys-1)** | 0 (392) | |
| skn-1(RNAi); tels98 | 16 (215) | |
| mom-1(or10) | 85§ | 83 (55) |
| mom-1(or10); sys-1(RNAi) | 93 (41) | |
| mom-1(or10); tels3 | 93 (41) | |
| mom-2(or42) | 73§ | 72 (105) |
| mom-2(RNAi) | 14‡‡ | 28 (49) |
| mom-2(or42); sys-1(RNAi) | 100 (229) | |
| mom-2(or42); teEx321 | 10 (39) | |
| mom-2(RNAi)††; tels3 | 60 (67) | |
| mom-2(or42); tels98 | 28 (145) | |
| mom-2(or42); tels98; skn-1(RNAi) | 94 (467) | |
| mom-3(or78) | 65§ | 70 (60) |
| mom-3(or78); sys-1(RNAi) | 81 (80) | |
| mom-3(or78); teEx321 | 13 (15) | |
| mom-3(or78); tels3 | 84 (50) | |
| mom-4(ne19) | 43‡‡ | 40 (65) |
| mom-4(ne19); sys-1(RNAi) | 100 (246) | |
| mom-4(ne19); teEx321 | 1 (67) | |
| mom-4(ne19); tels3 | 75 (56) | |
| mom-5(or57) | 5§ | 14 (113) |
| mom-5(or57); sys-1(RNAi) | 60 (134) | |
| mom-5(or57); teEx321 | 0 (62) | |
| mom-5(or57); tels3 | 65 (93) | |
| mom-5(or57); tels98 | 4 (131) | |
| mom-5(or57); tels98; skn-1(RNAi) | 52 (188) | |
| apr-1(RNAi) | 26‡‡ | 29 (70) |
| apr-1(RNAi); teEx321 | 0 (20) | |
| apr-1(RNAi); tels3 | 71 (62) | |
| lit-1(t1534) | 0§§ | 0 (116) |
| lit-1(t1534); sys-1(RNAi) | 100 (126) | |
| lit-1(t1534); tels3 | 94 (56) | |
| lit-1(1512) | 100§§ | 99 (224) |
| lit-1(t1512); teEx321 | 65 (31) | |
| wrm-1(RNAi) | 100‡‡ | 100 (122) |
| wrm-1(RNAi); sys-1(RNAi) | 100 (87) | |
| wrm-1(RNAi); teEx321 | 100 (143) | |
| wrm-1(RNAi); tels3 | 100 (170) | |
| med-1/2(RNAi)¶¶ | 7-52 | 16 (61) |
| med-1/2(RNAi); mom-2(or42) | 85 (66) | |
| med-1/2(RNAi); mom-4(ne19) | 46 (57) | |
| med-1/2(RNAi); mom-2(or42); teEx321 | 23 (31) | |
| med-1/2(RNAi); mom-4(ne19); teEx321 | 19 (27) |
(Bowerman et al., 1992).
Numbers indicate percentage gutless. Numbers in parentheses indicate total number of embryos scored.
All skn-1(RNAi) was performed by feeding, except those indicated as injection (inj).
(Thorpe et al., 1997).
teEx321 was crossed into adult mutant hermaphrodites and GFP-positive embryos were scored.
Balanced mom mutant strains carrying tels3 and tels98were generated. Embryos were collected from mutant hermaphrodites and scored for gut formation.
tels3 is on V, the same linkage group as mom-2, therefore mom-2(RNAi) was performed.
(Rocheleau et al.,1997).
(Kaletta et al., 1997).
7% gutless (Goszczynski et al.,2005). 52% gutless (Maduro et al., 2001).
6 mg/ml double-stranded med-1/2 RNA was injected and embryos laid 24 hours post-injection were assayed for gut formation.