Developmental features of wild-type and amp1, mp and amp1 mp mutant plants
| A. Shoot meristem size . | . | . | . | . |
|---|---|---|---|---|
| Measurement . | Ler . | amp1-9 . | amp1-9 mpG92 . | mpG92 . |
| Meristem size, 3 DAG | ||||
| Meristem area, μm2 | 356±28 (32) | 5548±551 (25)* | 5220±593 (29)* | 26±2 (49)* |
| Meristem width, μm | 47±2 (32) | 148±6 (25)* | 152±7(29)* | 14±3 (49)* |
| Meristem height, μm | 11±1 (32) | 54±4 (25)* | 52±4 (29)* | 3±1 (49)* |
| Meristem size, 5 DAG | ||||
| Meristem area, μm2 | 589±77 (28) | 9214±988 (18)* | 8766±902 (23)* | 211±23 (26)* |
| Meristem width, μm | 62±3 (28) | 196±7 (18)* | 190±9 (23)* | 40±3 (26)* |
| Meristem height, μm | 14±1 (28) | 70±6 (18)* | 68±6 (23)* | 8±2 (26)* |
| A. Shoot meristem size . | . | . | . | . |
|---|---|---|---|---|
| Measurement . | Ler . | amp1-9 . | amp1-9 mpG92 . | mpG92 . |
| Meristem size, 3 DAG | ||||
| Meristem area, μm2 | 356±28 (32) | 5548±551 (25)* | 5220±593 (29)* | 26±2 (49)* |
| Meristem width, μm | 47±2 (32) | 148±6 (25)* | 152±7(29)* | 14±3 (49)* |
| Meristem height, μm | 11±1 (32) | 54±4 (25)* | 52±4 (29)* | 3±1 (49)* |
| Meristem size, 5 DAG | ||||
| Meristem area, μm2 | 589±77 (28) | 9214±988 (18)* | 8766±902 (23)* | 211±23 (26)* |
| Meristem width, μm | 62±3 (28) | 196±7 (18)* | 190±9 (23)* | 40±3 (26)* |
| Meristem height, μm | 14±1 (28) | 70±6 (18)* | 68±6 (23)* | 8±2 (26)* |
| B. Frequency of adventitious root formation . | . | . | . | . | |
|---|---|---|---|---|---|
| Measurement . | Ler . | amp1-9 . | amp1-9 mpG92 . | mpG92 . | |
| No. adv roots, 14 DAG, dark | 0.2±0.1 (55) | 3.4±0.2 (51)† | 1.5±0.1 (46)† | 0 (39)† | |
| No. adv roots, 7 DAG, light | 1.0±0.6 (21) | 2.2±1.1 (26)† | 1.2±0.7 (45) | 0 (36)† | |
| B. Frequency of adventitious root formation . | . | . | . | . | |
|---|---|---|---|---|---|
| Measurement . | Ler . | amp1-9 . | amp1-9 mpG92 . | mpG92 . | |
| No. adv roots, 14 DAG, dark | 0.2±0.1 (55) | 3.4±0.2 (51)† | 1.5±0.1 (46)† | 0 (39)† | |
| No. adv roots, 7 DAG, light | 1.0±0.6 (21) | 2.2±1.1 (26)† | 1.2±0.7 (45) | 0 (36)† | |
| C. Early emergence of lateral roots is observed in amp1 seedlings at 4 DAG . | . | . | ||
|---|---|---|---|---|
| Genotype . | n . | Number of initiation sites . | ||
| WT | 39 | 0.1±0.05 | ||
| amp1-9 | 37 | 3.0±0.21‡ | ||
| amp1-9 mpG92 | 37 | 0.5±0.12‡ | ||
| mpG92 | 40 | 0±0 | ||
| C. Early emergence of lateral roots is observed in amp1 seedlings at 4 DAG . | . | . | ||
|---|---|---|---|---|
| Genotype . | n . | Number of initiation sites . | ||
| WT | 39 | 0.1±0.05 | ||
| amp1-9 | 37 | 3.0±0.21‡ | ||
| amp1-9 mpG92 | 37 | 0.5±0.12‡ | ||
| mpG92 | 40 | 0±0 | ||
| D. Restoration of seedling pattern (rooted seedlings with hypocotyls at 10 DAG) . | . | . | . | |||
|---|---|---|---|---|---|---|
| Genotype . | No. of rooting individuals . | Total (n) . | Frequency of rooting (%) . | |||
| amp1-1 mpBS1354 | 35 | 338 | 10.3 | |||
| amp1-1 mpG12 | 8 | 195 | 4.1 | |||
| amp1-1 mpG33 | 12 | 136 | 8.8 | |||
| amp1-11 mpBS1354 | 57 | 147 | 38.8 | |||
| amp1-10 mpG12 | 40 | 132 | 30.3 | |||
| amp1-8 mpG92 | 64 | 197 | 32.5 | |||
| amp1-9 mpG92 | 26 | 165 | 15.8 | |||
| D. Restoration of seedling pattern (rooted seedlings with hypocotyls at 10 DAG) . | . | . | . | |||
|---|---|---|---|---|---|---|
| Genotype . | No. of rooting individuals . | Total (n) . | Frequency of rooting (%) . | |||
| amp1-1 mpBS1354 | 35 | 338 | 10.3 | |||
| amp1-1 mpG12 | 8 | 195 | 4.1 | |||
| amp1-1 mpG33 | 12 | 136 | 8.8 | |||
| amp1-11 mpBS1354 | 57 | 147 | 38.8 | |||
| amp1-10 mpG12 | 40 | 132 | 30.3 | |||
| amp1-8 mpG92 | 64 | 197 | 32.5 | |||
| amp1-9 mpG92 | 26 | 165 | 15.8 | |||
| E. Floral organ numbers of amp1 mp double mutants . | . | . | . | . | . | . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total . | . | . | . | Sepals . | . | Petals . | . | Stamens . | . | Carpels . | . | |||||
| Genotype . | n . | Range . | Mean . | Range . | Mean . | Range . | Mean . | Range . | Mean . | Range . | Mean . | |||||
| Ler | 50 | 4 | 4.0 | 4 | 4.0 | 5-6 | 5.8±0.1 | 2 | 2.0 | 15-16 | 15.8±0.1 | |||||
| amp1-9 | 50 | 4 | 4.0 | 4 | 4.0 | 5-6 | 5.9±0.1 | 2 | 2.0 | 15-16 | 15.9±0.1 | |||||
| amp1-9 mpG92 | 40 | 0-4 | 2.4±0.1§ | 2-8 | 3.5±0.2§ | 1-4 | 2.7±0.2§ | 1-2 | 1.6±0.1§ | 7-15 | 10.2±0.2§ | |||||
| amp1-8 | 50 | 4 | 4.0 | 4 | 4.0 | 5-7 | 5.9±0.1 | 2 | 2.0 | 15-17 | 15.9±0.1 | |||||
| amp1-8 mpBS62 | 50 | 2-4 | 3.7±0.1§ | 2-4 | 3.6±0.1§ | 4-6 | 5.0±0.1§ | 1-2 | 1.9±0.1 | 11-16 | 14.2±0.2§ | |||||
| E. Floral organ numbers of amp1 mp double mutants . | . | . | . | . | . | . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total . | . | . | . | Sepals . | . | Petals . | . | Stamens . | . | Carpels . | . | |||||
| Genotype . | n . | Range . | Mean . | Range . | Mean . | Range . | Mean . | Range . | Mean . | Range . | Mean . | |||||
| Ler | 50 | 4 | 4.0 | 4 | 4.0 | 5-6 | 5.8±0.1 | 2 | 2.0 | 15-16 | 15.8±0.1 | |||||
| amp1-9 | 50 | 4 | 4.0 | 4 | 4.0 | 5-6 | 5.9±0.1 | 2 | 2.0 | 15-16 | 15.9±0.1 | |||||
| amp1-9 mpG92 | 40 | 0-4 | 2.4±0.1§ | 2-8 | 3.5±0.2§ | 1-4 | 2.7±0.2§ | 1-2 | 1.6±0.1§ | 7-15 | 10.2±0.2§ | |||||
| amp1-8 | 50 | 4 | 4.0 | 4 | 4.0 | 5-7 | 5.9±0.1 | 2 | 2.0 | 15-17 | 15.9±0.1 | |||||
| amp1-8 mpBS62 | 50 | 2-4 | 3.7±0.1§ | 2-4 | 3.6±0.1§ | 4-6 | 5.0±0.1§ | 1-2 | 1.9±0.1 | 11-16 | 14.2±0.2§ | |||||
| F. Apical patern phenotypes of amp1 mp double mutants . | . | . | . | . | . | |
|---|---|---|---|---|---|---|
| Genotype . | n . | Monocot % . | Fused % . | > 2 cots % . | Other % . | |
| amp1-11 mpBS1354 | 88 | 23.9 | 14.8 | 61.3 | ||
| amp1-8 mpBS62 | 181 | 2.7 | 3.9 | 92.8 | 0.6 | |
| amp1-9 mpG92 | 125 | 20.4 | 4.7 | 64.6 | 10.3 | |
| mpBS1354 | 92 | 50.0 | 4.3 | 44.6 | 1.1 | |
| mpBS62 | 74 | 35.1 | 8.1 | 56.8 | ||
| mpG92 | 107 | 14.9 | 25.3 | 59.8 | ||
| F. Apical patern phenotypes of amp1 mp double mutants . | . | . | . | . | . | |
|---|---|---|---|---|---|---|
| Genotype . | n . | Monocot % . | Fused % . | > 2 cots % . | Other % . | |
| amp1-11 mpBS1354 | 88 | 23.9 | 14.8 | 61.3 | ||
| amp1-8 mpBS62 | 181 | 2.7 | 3.9 | 92.8 | 0.6 | |
| amp1-9 mpG92 | 125 | 20.4 | 4.7 | 64.6 | 10.3 | |
| mpBS1354 | 92 | 50.0 | 4.3 | 44.6 | 1.1 | |
| mpBS62 | 74 | 35.1 | 8.1 | 56.8 | ||
| mpG92 | 107 | 14.9 | 25.3 | 59.8 | ||
Values are mean±s.e.m., (n). Note that only (13/49) and(10/26) mpG92 mutants produced shoot apical meristems (SAMs) at 3 days after germination (DAG) and 5 DAG, respectively. Mutants that failed to produce a SAM were included in the calculations and given a measurement value of zero since all mutants will form SAMs subsequently.
* Significantly (P<0.05) different from wild-type population, based on Student's t-test.
Values are mean number of adventitious roots from the hypocotyl±s.e.m., (n).
† Significantly (P<0.05) different from wild-type population, based on Student's t-test.
Results shown are the average number of lateral roots (initiation sites)marked by CycB1, 1::GUS expression in the primary root±s.e.m. at 4 DAG.
‡ Significantly (P<0.05) different from wild-type (WT) population, based on Student's t-test.
Values are range and mean number of floral organs produced±s.e.m.
§ Significantly (P<0.05) different from wild-type population, based on Student's t-test.