Sample ID* . | Protein . | Sequence coverage (%) . | Protein score† . | Swiss-Prot acc. no. . |
---|---|---|---|---|
1 | GRP78 | 30 | 100 | P11021 |
2 | Hsp70 | 35 | 140 | P08107 |
3 | Carbamoyl phosphate synthetase | 10 | 691 | Q6F8M7 |
4 | Hsp90 | 28 | 741 | P07900 |
5 | Hsp70 | 14 | 262 | P08107 |
6 | AMPKα | 43 | 1089 | P54645 |
7 | Tubulin | 41 | 675 | P05209 |
8 | Cdc37 | 15 | 219 | Q16543 |
9 | AMPKβ1 | 47 | 300 | Q9Y478 |
10 | AMPKγ1 | 43 | 391 | P54619 |
11 | AMPKβ2 | 51 | 385 | O43741 |
12 | Adenine nucleotide translocase 2 | 11 | 179 | PO5141 |
13 | Qsk | 28 | 244 | Q9Y2K2 |
14 | Qsk | 33 | 128 | Q9Y2K2 |
15 | Hsp70 | 37 | 248 | P08107 |
16 | Tubulin | 28 | 96 | P05209 |
17 | 14-3-3ζ | 40 | 178 | P63104 |
18 | 14-3-3ϵ | 42 | 121 | P62258 |
19 | Sik | 19 | 127 | Q86YJ2 |
20 | Hsp70 | 37 | 131 | P08107 |
21 | Sik | 25 | 116 | Q86YJ2 |
22 | Tubulin | 34 | 119 | P05209 |
23 | 14-3-3ζ | 30 | 98 | P63104 |
24 | 14-3-3ϵ | 38 | 120 | P62258 |
25 | Qik | 30 | 260 | Q9H0K1 |
26 | VCP | 33 | 210 | P55072 |
27 | Hsp70 | 52 | 369 | P08107 |
28 | PP2A reg. subunit A | 38 | 199 | Q8NHV8 |
29 | Tubulin | 24 | 98 | P05209 |
30 | PP2A reg. subunit B | 49 | 145 | P63151 |
31 | PP2A catalytic subunit | 49 | 203 | P67775 |
32 | USP9 | 22 | 448 | Q93008 |
33 | Nuak1 | 48 | 409 | O60285 |
34 | Hsp70 | 20 | 52 | P08107 |
35 | Nuak2 | 24 | 192 | Q9H093 |
36 | Hsp90 | 22 | 221 | P07900 |
37 | Hsp70 | 36 | 157 | P08107 |
38 | Nuak2 | 33 | 124 | Q9H093 |
39 | cdc37 | 16 | 92 | Q16543 |
40 | Brsk1 | 42 | 640 | Q8TDC2 |
41 | Hsp70 | 57 | 389 | P08107 |
42 | Brsk2 | 52 | 397 | Q8IWQ3 |
43 | Hsp70 | 39 | 256 | P08107 |
44 | Tubulin | 32 | 147 | P05209 |
45 | Mark1 | 34 | 176 | Q9P0L2 |
46 | Hsp70 | 33 | 111 | P08107 |
47 | Tubulin | 32 | 98 | P05209 |
48 | 14-3-3ζ | 25 | 83 | P63104 |
49 | 14-3-3ϵ | 34 | 140 | P62258 |
50 | Mark2 | 49 | 518 | Q15524 |
51 | Hsp70 | 42 | 120 | P08107 |
52 | 14-3-3ζ | 25 | 83 | P63104 |
53 | 14-3-3ϵ | 32 | 127 | P62258 |
54 | Mark3 | 34 | 178 | P27448 |
55 | Hsp70 | 25 | 83 | P08107 |
56 | 14-3-3ζ | 31 | 98 | P63104 |
57 | 14-3-3ϵ | 34 | 104 | P62258 |
58 | USP9 | 14 | 204 | Q93008 |
59 | Mark4 | 56 | 565 | Q96GZ3 |
60 | Hsp70 | 25 | 122 | P08107 |
61 | Tubulin | 25 | 98 | P05209 |
62 | 14-3-3ζ | 35 | 104 | P63104 |
63 | 14-3-3ϵ | 36 | 120 | P62258 |
64 | Snrk | 11 | 91 | Q9NRH2 |
65 | Hsp70 | 34 | 125 | P08107 |
Sample ID* . | Protein . | Sequence coverage (%) . | Protein score† . | Swiss-Prot acc. no. . |
---|---|---|---|---|
1 | GRP78 | 30 | 100 | P11021 |
2 | Hsp70 | 35 | 140 | P08107 |
3 | Carbamoyl phosphate synthetase | 10 | 691 | Q6F8M7 |
4 | Hsp90 | 28 | 741 | P07900 |
5 | Hsp70 | 14 | 262 | P08107 |
6 | AMPKα | 43 | 1089 | P54645 |
7 | Tubulin | 41 | 675 | P05209 |
8 | Cdc37 | 15 | 219 | Q16543 |
9 | AMPKβ1 | 47 | 300 | Q9Y478 |
10 | AMPKγ1 | 43 | 391 | P54619 |
11 | AMPKβ2 | 51 | 385 | O43741 |
12 | Adenine nucleotide translocase 2 | 11 | 179 | PO5141 |
13 | Qsk | 28 | 244 | Q9Y2K2 |
14 | Qsk | 33 | 128 | Q9Y2K2 |
15 | Hsp70 | 37 | 248 | P08107 |
16 | Tubulin | 28 | 96 | P05209 |
17 | 14-3-3ζ | 40 | 178 | P63104 |
18 | 14-3-3ϵ | 42 | 121 | P62258 |
19 | Sik | 19 | 127 | Q86YJ2 |
20 | Hsp70 | 37 | 131 | P08107 |
21 | Sik | 25 | 116 | Q86YJ2 |
22 | Tubulin | 34 | 119 | P05209 |
23 | 14-3-3ζ | 30 | 98 | P63104 |
24 | 14-3-3ϵ | 38 | 120 | P62258 |
25 | Qik | 30 | 260 | Q9H0K1 |
26 | VCP | 33 | 210 | P55072 |
27 | Hsp70 | 52 | 369 | P08107 |
28 | PP2A reg. subunit A | 38 | 199 | Q8NHV8 |
29 | Tubulin | 24 | 98 | P05209 |
30 | PP2A reg. subunit B | 49 | 145 | P63151 |
31 | PP2A catalytic subunit | 49 | 203 | P67775 |
32 | USP9 | 22 | 448 | Q93008 |
33 | Nuak1 | 48 | 409 | O60285 |
34 | Hsp70 | 20 | 52 | P08107 |
35 | Nuak2 | 24 | 192 | Q9H093 |
36 | Hsp90 | 22 | 221 | P07900 |
37 | Hsp70 | 36 | 157 | P08107 |
38 | Nuak2 | 33 | 124 | Q9H093 |
39 | cdc37 | 16 | 92 | Q16543 |
40 | Brsk1 | 42 | 640 | Q8TDC2 |
41 | Hsp70 | 57 | 389 | P08107 |
42 | Brsk2 | 52 | 397 | Q8IWQ3 |
43 | Hsp70 | 39 | 256 | P08107 |
44 | Tubulin | 32 | 147 | P05209 |
45 | Mark1 | 34 | 176 | Q9P0L2 |
46 | Hsp70 | 33 | 111 | P08107 |
47 | Tubulin | 32 | 98 | P05209 |
48 | 14-3-3ζ | 25 | 83 | P63104 |
49 | 14-3-3ϵ | 34 | 140 | P62258 |
50 | Mark2 | 49 | 518 | Q15524 |
51 | Hsp70 | 42 | 120 | P08107 |
52 | 14-3-3ζ | 25 | 83 | P63104 |
53 | 14-3-3ϵ | 32 | 127 | P62258 |
54 | Mark3 | 34 | 178 | P27448 |
55 | Hsp70 | 25 | 83 | P08107 |
56 | 14-3-3ζ | 31 | 98 | P63104 |
57 | 14-3-3ϵ | 34 | 104 | P62258 |
58 | USP9 | 14 | 204 | Q93008 |
59 | Mark4 | 56 | 565 | Q96GZ3 |
60 | Hsp70 | 25 | 122 | P08107 |
61 | Tubulin | 25 | 98 | P05209 |
62 | 14-3-3ζ | 35 | 104 | P63104 |
63 | 14-3-3ϵ | 36 | 120 | P62258 |
64 | Snrk | 11 | 91 | Q9NRH2 |
65 | Hsp70 | 34 | 125 | P08107 |
The colloidal Coomassie-Blue-stained bands that were labelled as indicated in Fig. 1C were excised from the gel, digested in-gel with trypsin, and their identities were determined by tryptic peptide mass-spectral fingerprint as described in Materials and Methods.
Mascot protein score where a value >63 is considered significant (P<0.05).