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Table 1.

Sequences of DNA probes and competitors used for the EMSA with HLH-2 and LAG-1

EMSA with HLH-2

DNA probe containing wild-type E-boxes

gaattcaaaaattccttaCACCTGtctttccacctttcccgctccccccgtccattaccactttgttaaccttgggggcacactagtaaccattacattaggatttacaaatcggaaCACCTGgtgcttggtgaattc

DNA probe containing mutated E-boxes

gaattcaaaaattccttaCTCCTAtctttccacctttcccgctccccccgtccattaccactttgttaaccttgggggcacactagtaaccattacattaggatttacaaatcggaaCTCCTAgtgcttggtgaattc

Wild-type competitor DNAs

Mutated competitor DNAs

1^st: aaattccttaCACCTGtctttccacc

1^st: aaattccttaCTCCTAtctttccacc

2^nd: caaatcggaaCACCTGgtgccttggt

2^nd: caaatcggaaCTCCTAgtgccttggt

EMSA with LAG-1

DNA probe containing wild-type LAG-1 binding sites

gaattctcgcgactCGTGGGAAaatgggcggaagggcacCGTGGGAAaatagttccaggaattc

Wild-type competitor DNAs

Mutated competitor DNAs

1^st: taataatctctTCCCAaactacacctc

1^st: taataatctctAACCAaactacacctc

2^nd: cctaactcctaTCCCAaattcgtacat

2^nd: cctaactcctaAACCAaattcgtacat

3^rd: ttcttgtttctTCCCAcgtacagtttc

3^rd: ttcttgtttctAACCAcgtacagtttc

4^th: ttacaaattctTCCCAgaacccaaaac

4^th: ttacaaattctAACCAgaacccaaaac

5^th: gggatccgagaTGGGAaaagagactcg

5^th: gggatccgagaTGGTTaaagagactcg

6^th: acagtcgctgaTGGGAaaatggataat

6^th: acagtcgctgaTGGTTaaatggataat

7^th: tgctctcacagTGGGAaaattagattc

7^th: tgctctcacagTGGTTaaattagattc

8^th: gcgaagatctgTGGGAaaatatacaga

8^th: gcgaagatctgTGGTTaaatatacaga

Sequences of DNA probes for HLH-2 and competitors for HLH-2 and LAG-1 (see Fig. 6) from the C. elegans egl-43 gene. The DNA probe sequence for LAG-1 EMSA has been used for EMSA with CBF-1 and Su(H) proteins, mammalian and D. melanogasterorthologs of LAG-1 (Zimber-Strobl et al.,1994). Sequences of the upper strand of DNA duplexes are shown. Bold characters represent wild-type or mutated E-boxes and LAG-1 binding sites.

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