Effect of reducing Gdnf gene dosage on kidney agenesis in Itga8-null mice
n . | Genotype . | Number of kidneys observed (percent agenesis) . |
|---|---|---|
| 14 | Itga8+/− | 28 (0%) |
| 17 | Gdnf+/− | 31 (9%) |
| 17 | Itga8+/−;Gdnf+/− | 16 (53%)** |
| 11 | Itga8+/− | 22 (0%) |
| 11 | Itga8−/− | 9 (59%) |
| 11 | Itga8−/−;Gdnf+/− | 1 (95%)** |
n . | Genotype . | Number of kidneys observed (percent agenesis) . |
|---|---|---|
| 14 | Itga8+/− | 28 (0%) |
| 17 | Gdnf+/− | 31 (9%) |
| 17 | Itga8+/−;Gdnf+/− | 16 (53%)** |
| 11 | Itga8+/− | 22 (0%) |
| 11 | Itga8−/− | 9 (59%) |
| 11 | Itga8−/−;Gdnf+/− | 1 (95%)** |
Kidneys were dissected from newborn progeny derived from the following crosses: top, Itga8+/− × Gdnf+/−; bottom, Itga8+/−× Itga8+/−;Gdnf+/−. Note that the Gdnf-null allele used in these crosses was GdnflacZ (Moore et al., 1996). Here n is the number of mice examined. The percentage of agenesis was determined as described in the legend to Fig. 2F. Significance was determined using Student's t-test, with n as the number of kidneys and the mean as the percentage of agenesis. **P<0.005.