A. Real time PCR primers . | . | . |
---|---|---|
Genes . | Positions (bp) . | Sequences . |
Apolipoprotein J | 937-959 | 5′-gga tga agg acc agt gtg aca ag-3′ |
1032-1050 | 5′-cag cga cct gga ggg att c-3′ | |
Fibronectin | 5023-5041 | 5′-tgt ggt tgc ctt gca cga t-3′ |
5111-5131 | 5′-gct tgt ggg tgt gac ctg agt-3′ | |
Osteonectin | 868-889 | 5′-gag acc tgt gac ctg gac aat g-3′ |
957-982 | 5′-gga agg agt gga ttt aga tca caa ga-3′ | |
SM22 | 511-530 | 5′-cgt gga gat ccc aac tgg tt-3′ |
586-606 | 5′-aag gcc aat gac atg ctt tcc-3′ | |
p21WAF-1 | 495-515 | 5′-ctg gag act ctc agg gtc gaa-3′ |
599-617 | 5′-cca gga ctg cag gct tcc t-3′ | |
p53 | 1209-1229 | 5′-aag aaacca ctg gat gga gaa-3′ |
1263-1283 | 5′-cag ctc tcg gaa cat ctc gaa-3′ | |
c-fos | 249-268 | 5′-tca ccc gca gac tcc ttc tc-3′ |
323-343 | 5′-gtg gga atg aag ttg gca ctg-3′ | |
c-jun | 2016-2033 | 5′-gga tca agg cgg aga gga a-3′ |
2088-2102 | 5′-tcc agc cgg gcg att-3′ | |
TGF-β1 | 1788-1808 | 5′-agg gct acc atg cca act tct-3′ |
1869-1889 | 5′-ccg ggt tat gct ggt tgt aca-3′ | |
TGF-β2 | 1067-1083 | 5′-acc aac cgg cgg aag aa-3′ |
1153-1176 | 5′-cac cct aga tcc ctc ttg aaa tca-3′ | |
TGF-β3 | 1288-1305 | 5′-agg ccc ttg ccc ata cct-3′ |
1349-1372 | 5′-aga tgc ttc agg gtt cag agt gtt-3′ | |
GAPDH | 942-963 | 5′-acc cac tcc tcc acc ttt gac-3′ |
1033-1053 | 5′-gtc cac cac cct gtt gct gta-3′ |
A. Real time PCR primers . | . | . |
---|---|---|
Genes . | Positions (bp) . | Sequences . |
Apolipoprotein J | 937-959 | 5′-gga tga agg acc agt gtg aca ag-3′ |
1032-1050 | 5′-cag cga cct gga ggg att c-3′ | |
Fibronectin | 5023-5041 | 5′-tgt ggt tgc ctt gca cga t-3′ |
5111-5131 | 5′-gct tgt ggg tgt gac ctg agt-3′ | |
Osteonectin | 868-889 | 5′-gag acc tgt gac ctg gac aat g-3′ |
957-982 | 5′-gga agg agt gga ttt aga tca caa ga-3′ | |
SM22 | 511-530 | 5′-cgt gga gat ccc aac tgg tt-3′ |
586-606 | 5′-aag gcc aat gac atg ctt tcc-3′ | |
p21WAF-1 | 495-515 | 5′-ctg gag act ctc agg gtc gaa-3′ |
599-617 | 5′-cca gga ctg cag gct tcc t-3′ | |
p53 | 1209-1229 | 5′-aag aaacca ctg gat gga gaa-3′ |
1263-1283 | 5′-cag ctc tcg gaa cat ctc gaa-3′ | |
c-fos | 249-268 | 5′-tca ccc gca gac tcc ttc tc-3′ |
323-343 | 5′-gtg gga atg aag ttg gca ctg-3′ | |
c-jun | 2016-2033 | 5′-gga tca agg cgg aga gga a-3′ |
2088-2102 | 5′-tcc agc cgg gcg att-3′ | |
TGF-β1 | 1788-1808 | 5′-agg gct acc atg cca act tct-3′ |
1869-1889 | 5′-ccg ggt tat gct ggt tgt aca-3′ | |
TGF-β2 | 1067-1083 | 5′-acc aac cgg cgg aag aa-3′ |
1153-1176 | 5′-cac cct aga tcc ctc ttg aaa tca-3′ | |
TGF-β3 | 1288-1305 | 5′-agg ccc ttg ccc ata cct-3′ |
1349-1372 | 5′-aga tgc ttc agg gtt cag agt gtt-3′ | |
GAPDH | 942-963 | 5′-acc cac tcc tcc acc ttt gac-3′ |
1033-1053 | 5′-gtc cac cac cct gtt gct gta-3′ |
B. Primers used for the detection of the common 4977 bp mitochondrial DNA deletion . | . | . |
---|---|---|
Primers . | Positions (bp) . | Sequences . |
H1 | 219-238 | 5′-atg ctt gta gga cat aat aa-3′ |
L1 | 447-466 | 5′-agt ggg agg gga aaa taa ta-3′ |
H2 | 8150-8169 | 5′-ccg ggg gta tac tac ggt ca-3′ |
L2 | 13631-13650 | 5′-ggg gaa gcg agg ttg acc tg-3′ |
H3 | 8197-8215 | 5′-cag ttt cat gcc cat cgt c-3′ |
L3 | 13560-13578 | 5′-gat gag agt aat aga tag g-3′ |
B. Primers used for the detection of the common 4977 bp mitochondrial DNA deletion . | . | . |
---|---|---|
Primers . | Positions (bp) . | Sequences . |
H1 | 219-238 | 5′-atg ctt gta gga cat aat aa-3′ |
L1 | 447-466 | 5′-agt ggg agg gga aaa taa ta-3′ |
H2 | 8150-8169 | 5′-ccg ggg gta tac tac ggt ca-3′ |
L2 | 13631-13650 | 5′-ggg gaa gcg agg ttg acc tg-3′ |
H3 | 8197-8215 | 5′-cag ttt cat gcc cat cgt c-3′ |
L3 | 13560-13578 | 5′-gat gag agt aat aga tag g-3′ |
C. Primers used to construct the different apo J retroviral expression vectors . | . |
---|---|
Primers . | Sequences . |
APOJ31F | 5′-cgc gga tcc gcg gcg acc atg atg aag act ctg ctg ctg tt-3′ |
APOJ31R | 5′-tgc tct aga gcc ctc ctc ccg gtg ctt ttt gcg-3′ |
APOJPNF | 5′-g ggt taa ccc gcc acc atg atg aag act ctg ctg ctg tt-3′ |
APOJPNR | 5′-c ggg atc ccg gcg ggt tta aac tca atg gtg-3′ |
TRAPOJR | 5′-c ggg atc ccg tca ctc ctc ccg gtg ctt ttt gc-3′ |
C. Primers used to construct the different apo J retroviral expression vectors . | . |
---|---|
Primers . | Sequences . |
APOJ31F | 5′-cgc gga tcc gcg gcg acc atg atg aag act ctg ctg ctg tt-3′ |
APOJ31R | 5′-tgc tct aga gcc ctc ctc ccg gtg ctt ttt gcg-3′ |
APOJPNF | 5′-g ggt taa ccc gcc acc atg atg aag act ctg ctg ctg tt-3′ |
APOJPNR | 5′-c ggg atc ccg gcg ggt tta aac tca atg gtg-3′ |
TRAPOJR | 5′-c ggg atc ccg tca ctc ctc ccg gtg ctt ttt gc-3′ |