Primers used in this study
| Primers used for genotyping, cloning and expression analyses | |||
| P1 | 5′-GTGATCACTGTTCTCAACCAGCT-3′ | ||
| P2 | 5′-TGGTATATTGTCGGTGTTTACAT-3′ | ||
| P3 | 5′-CGGAATTCGTTGTGATGGCGAGAGAAAAGATTC-3′ | ||
| P4 | 5′-CGGGATCCTTCCATCTCTAACCACCATACGGT-3′ | ||
| P5 | 5′-CAAGGACTTGACATTGAAGAGCTTCA-3′ | ||
| P6 | 5′-CTGATCTCACTCATAATCTTGTCAC-3′ | ||
| P7 | 5′-AGCTGCAGAAAACGAGAAGCTCTCTG-3′ | ||
| P8 | 5′-GGGCTACTCTCTTCATCACCTCTTCC-3′ | ||
| P9 | 5′-GAAGCAGCAAACATGATGAAG-3′ | ||
| P10 | 5′-TTGCGTCTCTACTTCAGAACTTGGG-3′ | ||
| P11 | 5′-GTGACAAGATTATGAGTGAGATCAG-3′ | ||
| P12 | 5′-GAATTCACTACTTAGACATTGTCTC-3′ | ||
| P13 | 5′-ATCCGTGAAGAGTACCCAGAT-3′ | ||
| P14 | 5′-AAGAACCATGCACTCATCAGC-3′ | ||
| P15 | 5′-GAGGCTTTGGAGACAGAGTCGGTGA-3′ | ||
| P16 | 5′-AGATGGAAGCCCAAGCTTCAGGGAA-3′ | ||
| P17 | 5′-AACTGCAGTCGTTCCTTATAGCGGTGGAT-3′ | ||
| P18 | 5′-GGACTAGTTTCCCAAGATGGAAGCCTAACCAAC-3′ | ||
| P19 | 5′-ACAAGAGTTTTGTAATTTTCGGCCCATTATTCTCA-3′ | ||
| P20 | 5′-TGAGAATAATGGGCCGAAAATTACAAAACTCTTGT-3′ | ||
| Primer pairs used for ChIP assays | |||
| AGL24-1 | 5′-ACAAGTTCGAAATTTGGGCCA-3′ | 5′-TTCACGTTTTACCATTTGCCGT-3′ | |
| AGL24-2 | 5′-TGCTGTTCATCAGTTCATCTACC-3′ | 5′-CTTATCAGGTGTCGCATCTAG-3′ | |
| AGL24-3 | 5′-ATCCCCAATCATACCAAGTGAC-3′ | 5′-GTACTGGGAAATAAGAGAGCAG-3′ | |
| AGL24-4 | 5′-AGTTCAATCCATCAAGATCCTCTC-3′ | 5′-TCTTTGGTAGACCTACTGAACA-3′ | |
| SVP-1 | 5′-ATGGGTTTGTAGTAGTTGCGTGGAGTA-3′ | 5′-TTGGGACACGATCCATTGTCCGTACAG-3′ | |
| SVP-2 | 5′-TTCAGTGATGATTGATACCCCC-3′ | 5′-CACTAATTTGGAAAGTTTGTCATGC-3′ | |
| SVP-3 | 5′-TCCATTTCAGTCGTCTTGTCAC-3′ | 5′-GAAGAGATGGAGGAGGAGGAAG-3′ | |
| SVP-4 | 5′-CTGATACATAGGAGTTTACTGTATC-3′ | 5′-GAATATTACCGTAGTTAGATACC-3′ | |
| SVP-5 | 5′-GATCAACCACTATCATTTTCTAACTG-3′ | 5′-TCTAGCTGCTGAAGCTCTTCAATGTC-3′ | |
| SOC1-1 | 5′-TATATCGGGAGGAGGACCACAC-3′ | 5′-ATCCATACAGATTTTCGGACCT-3′ | |
| SOC1-2 | 5′-TCTCGTACCTATATGCCCCCACT-3′ | 5′-TTTATCTGTTGGGATGGAAAGA-3′ | |
| SOC1-3 | 5′-GCAAAAGAAGTAGCTTTCCTCG-3′ | 5′-AGCAGAGAGAGAAGAGACGAGTG-3′ | |
| SOC1-4 | 5′-GGATGCAACCTCCTTTCATGAG-3′ | 5′-ATATGGGTTTGGTTTCATTTGG-3′ | |
| SOC1-5 | 5′-ATCACATCTCTTTGACGTTTGCTT-3′ | 5′-GCCCTAATTTTGCAGAAACCAA-3′ | |
| SOC1-6 | 5′-TGTTTCAGACATTTGGTCCATTTG-3′ | 5′-AGTCTTGTACTTTTTCCCCCTATTTTAG-3′ | |
| ACTIN | 5′-CGTTTCGCTTTCCTTAGTGTTAGCT-3′ | 5′-AGCGAACGGATCTAGAGACTCACCTTG-3′ | |
| TUB2 | 5′-ATCCGTGAAGAGTACCCAGAT-3′ | 5′-AAGAACCATGCACTCATCAGC-3′ | |
| Primers used for genotyping, cloning and expression analyses | |||
| P1 | 5′-GTGATCACTGTTCTCAACCAGCT-3′ | ||
| P2 | 5′-TGGTATATTGTCGGTGTTTACAT-3′ | ||
| P3 | 5′-CGGAATTCGTTGTGATGGCGAGAGAAAAGATTC-3′ | ||
| P4 | 5′-CGGGATCCTTCCATCTCTAACCACCATACGGT-3′ | ||
| P5 | 5′-CAAGGACTTGACATTGAAGAGCTTCA-3′ | ||
| P6 | 5′-CTGATCTCACTCATAATCTTGTCAC-3′ | ||
| P7 | 5′-AGCTGCAGAAAACGAGAAGCTCTCTG-3′ | ||
| P8 | 5′-GGGCTACTCTCTTCATCACCTCTTCC-3′ | ||
| P9 | 5′-GAAGCAGCAAACATGATGAAG-3′ | ||
| P10 | 5′-TTGCGTCTCTACTTCAGAACTTGGG-3′ | ||
| P11 | 5′-GTGACAAGATTATGAGTGAGATCAG-3′ | ||
| P12 | 5′-GAATTCACTACTTAGACATTGTCTC-3′ | ||
| P13 | 5′-ATCCGTGAAGAGTACCCAGAT-3′ | ||
| P14 | 5′-AAGAACCATGCACTCATCAGC-3′ | ||
| P15 | 5′-GAGGCTTTGGAGACAGAGTCGGTGA-3′ | ||
| P16 | 5′-AGATGGAAGCCCAAGCTTCAGGGAA-3′ | ||
| P17 | 5′-AACTGCAGTCGTTCCTTATAGCGGTGGAT-3′ | ||
| P18 | 5′-GGACTAGTTTCCCAAGATGGAAGCCTAACCAAC-3′ | ||
| P19 | 5′-ACAAGAGTTTTGTAATTTTCGGCCCATTATTCTCA-3′ | ||
| P20 | 5′-TGAGAATAATGGGCCGAAAATTACAAAACTCTTGT-3′ | ||
| Primer pairs used for ChIP assays | |||
| AGL24-1 | 5′-ACAAGTTCGAAATTTGGGCCA-3′ | 5′-TTCACGTTTTACCATTTGCCGT-3′ | |
| AGL24-2 | 5′-TGCTGTTCATCAGTTCATCTACC-3′ | 5′-CTTATCAGGTGTCGCATCTAG-3′ | |
| AGL24-3 | 5′-ATCCCCAATCATACCAAGTGAC-3′ | 5′-GTACTGGGAAATAAGAGAGCAG-3′ | |
| AGL24-4 | 5′-AGTTCAATCCATCAAGATCCTCTC-3′ | 5′-TCTTTGGTAGACCTACTGAACA-3′ | |
| SVP-1 | 5′-ATGGGTTTGTAGTAGTTGCGTGGAGTA-3′ | 5′-TTGGGACACGATCCATTGTCCGTACAG-3′ | |
| SVP-2 | 5′-TTCAGTGATGATTGATACCCCC-3′ | 5′-CACTAATTTGGAAAGTTTGTCATGC-3′ | |
| SVP-3 | 5′-TCCATTTCAGTCGTCTTGTCAC-3′ | 5′-GAAGAGATGGAGGAGGAGGAAG-3′ | |
| SVP-4 | 5′-CTGATACATAGGAGTTTACTGTATC-3′ | 5′-GAATATTACCGTAGTTAGATACC-3′ | |
| SVP-5 | 5′-GATCAACCACTATCATTTTCTAACTG-3′ | 5′-TCTAGCTGCTGAAGCTCTTCAATGTC-3′ | |
| SOC1-1 | 5′-TATATCGGGAGGAGGACCACAC-3′ | 5′-ATCCATACAGATTTTCGGACCT-3′ | |
| SOC1-2 | 5′-TCTCGTACCTATATGCCCCCACT-3′ | 5′-TTTATCTGTTGGGATGGAAAGA-3′ | |
| SOC1-3 | 5′-GCAAAAGAAGTAGCTTTCCTCG-3′ | 5′-AGCAGAGAGAGAAGAGACGAGTG-3′ | |
| SOC1-4 | 5′-GGATGCAACCTCCTTTCATGAG-3′ | 5′-ATATGGGTTTGGTTTCATTTGG-3′ | |
| SOC1-5 | 5′-ATCACATCTCTTTGACGTTTGCTT-3′ | 5′-GCCCTAATTTTGCAGAAACCAA-3′ | |
| SOC1-6 | 5′-TGTTTCAGACATTTGGTCCATTTG-3′ | 5′-AGTCTTGTACTTTTTCCCCCTATTTTAG-3′ | |
| ACTIN | 5′-CGTTTCGCTTTCCTTAGTGTTAGCT-3′ | 5′-AGCGAACGGATCTAGAGACTCACCTTG-3′ | |
| TUB2 | 5′-ATCCGTGAAGAGTACCCAGAT-3′ | 5′-AAGAACCATGCACTCATCAGC-3′ | |