siRNA . | . | Control . | Nubp1 . | |
---|---|---|---|---|
Number of mitotic cells counted | 310 | 313 | ||
Cells with | 2 centrosomes | 268 (86.5%) | 229 (73.2%) | |
3 centrosomes | 15 (4.8%) | 35 (11.2%) | ||
4 centrosomes | 13 (4.2%) | 23 (7.3%) | ||
5 centrosomes | 7 (2.3%) | 6 (1.9%) | ||
6 centrosomes | – | 10 (3.2%) | ||
7 centrosomes | 4 (1.3%) | 2 (0.6%) | ||
8 centrosomes | 2 (0.6%) | 6 (1.9%) | ||
9 centrosomes | – | – | ||
⩾ 10 centrosomes | 1 (0.3%) | 2 (0.6%) | ||
Cells with multiple centrosomes (% total) | 42 (13.5%) | 84 (26.8%) | ||
Average number of centrosomes per cell (% of mitotic cells counted) | 2.34 | 2.67 |
siRNA . | . | Control . | Nubp1 . | |
---|---|---|---|---|
Number of mitotic cells counted | 310 | 313 | ||
Cells with | 2 centrosomes | 268 (86.5%) | 229 (73.2%) | |
3 centrosomes | 15 (4.8%) | 35 (11.2%) | ||
4 centrosomes | 13 (4.2%) | 23 (7.3%) | ||
5 centrosomes | 7 (2.3%) | 6 (1.9%) | ||
6 centrosomes | – | 10 (3.2%) | ||
7 centrosomes | 4 (1.3%) | 2 (0.6%) | ||
8 centrosomes | 2 (0.6%) | 6 (1.9%) | ||
9 centrosomes | – | – | ||
⩾ 10 centrosomes | 1 (0.3%) | 2 (0.6%) | ||
Cells with multiple centrosomes (% total) | 42 (13.5%) | 84 (26.8%) | ||
Average number of centrosomes per cell (% of mitotic cells counted) | 2.34 | 2.67 |
The average number of centrosomes per cell is significantly higher between silencing and control treatment (single-factor ANOVA with replication, P=0.0035).