siRNA . | . | Control . | KIFC5A . | |
---|---|---|---|---|
Number of mitotic cells counted | 802 | 209 | ||
Cells with | 2 centrosomes | 678 (84.5%) | 83 (39.7%) | |
3 centrosomes | 78 (9.7%) | 52 (24.9%) | ||
4 centrosomes | 33 (4.1%) | 26 (12.4%) | ||
5 centrosomes | 6 (0.7%) | 18 (8.6%) | ||
6 centrosomes | 3 (0.4%) | 14 (6.7%) | ||
7 centrosomes | 2 (0.2%) | 7 (3.3%) | ||
8 centrosomes | – | 4 (1.9%) | ||
9 centrosomes | 2 (0.2%) | 2 (1%) | ||
⩾10 centrosomes | – | 3 (1.4%) | ||
Cells with multiple centrosomes (% total) | 124 (15.5%) | 126 (60.3%) | ||
Average number of centrosomes per cell (% of mitotic cells counted) | 2.25 | 3.49 |
siRNA . | . | Control . | KIFC5A . | |
---|---|---|---|---|
Number of mitotic cells counted | 802 | 209 | ||
Cells with | 2 centrosomes | 678 (84.5%) | 83 (39.7%) | |
3 centrosomes | 78 (9.7%) | 52 (24.9%) | ||
4 centrosomes | 33 (4.1%) | 26 (12.4%) | ||
5 centrosomes | 6 (0.7%) | 18 (8.6%) | ||
6 centrosomes | 3 (0.4%) | 14 (6.7%) | ||
7 centrosomes | 2 (0.2%) | 7 (3.3%) | ||
8 centrosomes | – | 4 (1.9%) | ||
9 centrosomes | 2 (0.2%) | 2 (1%) | ||
⩾10 centrosomes | – | 3 (1.4%) | ||
Cells with multiple centrosomes (% total) | 124 (15.5%) | 126 (60.3%) | ||
Average number of centrosomes per cell (% of mitotic cells counted) | 2.25 | 3.49 |
The average number of centrosomes is significantly higher between silencing and control treatment (single-factor ANOVA with replication, P=0.006).