Effect of KIFC5A overexpression in NIH 3T3 cells
| . | Control transfection (YFP-alone vector) . | YFP-KIFC5A transfection . |
|---|---|---|
| Transfected cells counted | 2001 | 2205 |
| Number of cells in mitosis of which: | 75 (3.7% of total) | 41 (1.9% of total) |
| normal prophase/prometaphase | 10 (13.3% of mitotic) | 2 (4.88% of mitotic) |
| stalled prometaphase* | 2 (2.7% of mitotic) | 23 (56% of mitotic) |
| metaphase | 3 (4% of mitotic) | 1 (2.4% of mitotic) |
| anaphase | 6 (8% of mitotic) | 1 (2.4% of mitotic) |
| telophase | 54 (72% of mitotic) | 14 (34.1% of mitotic) |
| . | Control transfection (YFP-alone vector) . | YFP-KIFC5A transfection . |
|---|---|---|
| Transfected cells counted | 2001 | 2205 |
| Number of cells in mitosis of which: | 75 (3.7% of total) | 41 (1.9% of total) |
| normal prophase/prometaphase | 10 (13.3% of mitotic) | 2 (4.88% of mitotic) |
| stalled prometaphase* | 2 (2.7% of mitotic) | 23 (56% of mitotic) |
| metaphase | 3 (4% of mitotic) | 1 (2.4% of mitotic) |
| anaphase | 6 (8% of mitotic) | 1 (2.4% of mitotic) |
| telophase | 54 (72% of mitotic) | 14 (34.1% of mitotic) |
The percentage of cells in stalled prometaphase in YFP-KIFC5A-expressing cells is significantly different than in the control population (single-factor ANOVA with replication, P=1.67×10–4). The overall distribution of cells in distinct phases of mitosis is significantly different in overexpressing and control samples (multiple-factor ANOVA with replication, P=2.11×10–10).
See Fig. 5