Disruption of cell adhesion or the extracellular matrix results in EMEs
| . | Genotype* . | Description . | n . | EMEs† . | Pvalue‡ . | Pvalue‡ . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A screen of 847 Unc and Prz genes identifies components of the integrin complex and the ECM | ||||||||||||
| 1 | Φ(RNAi) | Wild type | 157 | 0.6±0.7 | – | – | ||||||
| 2 | pat-4(RNAi) | Integrin-linked kinase | 36 | 2.9±1.9 | <0.001 (1) | – | ||||||
| 3 | pat-6(RNAi) | Actopaxin | 43 | 3.0±2.0 | <0.001 (1) | – | ||||||
| 4 | lam-1(RNAi) | β-Laminin | 40 | 4.1±1.7 | <0.001 (1) | – | ||||||
| 5 | lam-2(RNAi) | γ-Laminin | 38 | 4.4±2.8 | <0.001 (1) | – | ||||||
| 6 | epi-1(RNAi) | αB-laminin | 86 | 3.2±1.7 | <0.001 (1) | – | ||||||
| 7 | pat-2(RNAi) | α-Integrin | 88 | 3.5±2.5 | <0.001 (1) | – | ||||||
| 8 | ina-1(RNAi) | α-Integrin | 88 | 0.5±0.7 | >0.05 (1) | – | ||||||
| 9 | unc-52(RNAi) | Perlecan | 44 | 1.8±1.2 | <0.001 (1) | – | ||||||
| 10 | lam-1(rh219) | β-Laminin (hypomorph) | 40 | 1.7±1.6 | <0.001 (1) | – | ||||||
| 11 | epi-1(rh152) | αB-laminin (hypomorph) | 44 | 3.0±2.4 | <0.001 (1) | – | ||||||
| 12 | ina-1(gm144) | α-Integrin (hypomorph) | 44 | 0.6±0.9 | >0.05 (1) | – | ||||||
| clr-1 loss of function and let-60 gain of function suppresses the EME phenotype of pat-2 and the laminin complex | ||||||||||||
| 13 | clr-1(e1745) 20°C | RPTP (hypomorph) | 21 | 0.6±2.5 | – | – | ||||||
| 15 | clr-1(e1745); lam-1(RNAi) | 58 | 1.0±1.1 | <0.001 (4) | <0.001 (13) | |||||||
| 14 | clr-1(e1745); lam-2(RNAi) | 60 | 1.0±1.2 | <0.001 (5) | <0.001 (13) | |||||||
| 16 | clr-1(e1745); epi-1(RNAi) | 48 | 0.7±0.8 | <0.001 (6) | >0.05 (13) | |||||||
| 17 | clr-1(e1745); pat-2(RNAi) | 61 | 0.7±1.0 | <0.001 (7) | >0.05 (13) | |||||||
| 18 | let-60(n1046) | Ras GTPase (gain of function) | 44 | 0.8±0.8 | – | – | ||||||
| 19 | let-60(n1046); lam-1(RNAi) | 27 | 3.1±1.0 | <0.001 (4) | <0.001 (18) | |||||||
| 20 | let-60(n1046); lam-2(RNAi) | 25 | 3.0±1.6 | <0.001 (5) | <0.001 (18) | |||||||
| 21 | let-60(n1046); epi-1(RNAi) | 60 | 2.6±1.6 | <0.001 (6) | <0.001 (18) | |||||||
| 22 | let-60(n1046); pat-2(RNAi) | 71 | 2.3±2.2 | <0.001 (7) | <0.001 (18) | |||||||
| . | Genotype* . | Description . | n . | EMEs† . | Pvalue‡ . | Pvalue‡ . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A screen of 847 Unc and Prz genes identifies components of the integrin complex and the ECM | ||||||||||||
| 1 | Φ(RNAi) | Wild type | 157 | 0.6±0.7 | – | – | ||||||
| 2 | pat-4(RNAi) | Integrin-linked kinase | 36 | 2.9±1.9 | <0.001 (1) | – | ||||||
| 3 | pat-6(RNAi) | Actopaxin | 43 | 3.0±2.0 | <0.001 (1) | – | ||||||
| 4 | lam-1(RNAi) | β-Laminin | 40 | 4.1±1.7 | <0.001 (1) | – | ||||||
| 5 | lam-2(RNAi) | γ-Laminin | 38 | 4.4±2.8 | <0.001 (1) | – | ||||||
| 6 | epi-1(RNAi) | αB-laminin | 86 | 3.2±1.7 | <0.001 (1) | – | ||||||
| 7 | pat-2(RNAi) | α-Integrin | 88 | 3.5±2.5 | <0.001 (1) | – | ||||||
| 8 | ina-1(RNAi) | α-Integrin | 88 | 0.5±0.7 | >0.05 (1) | – | ||||||
| 9 | unc-52(RNAi) | Perlecan | 44 | 1.8±1.2 | <0.001 (1) | – | ||||||
| 10 | lam-1(rh219) | β-Laminin (hypomorph) | 40 | 1.7±1.6 | <0.001 (1) | – | ||||||
| 11 | epi-1(rh152) | αB-laminin (hypomorph) | 44 | 3.0±2.4 | <0.001 (1) | – | ||||||
| 12 | ina-1(gm144) | α-Integrin (hypomorph) | 44 | 0.6±0.9 | >0.05 (1) | – | ||||||
| clr-1 loss of function and let-60 gain of function suppresses the EME phenotype of pat-2 and the laminin complex | ||||||||||||
| 13 | clr-1(e1745) 20°C | RPTP (hypomorph) | 21 | 0.6±2.5 | – | – | ||||||
| 15 | clr-1(e1745); lam-1(RNAi) | 58 | 1.0±1.1 | <0.001 (4) | <0.001 (13) | |||||||
| 14 | clr-1(e1745); lam-2(RNAi) | 60 | 1.0±1.2 | <0.001 (5) | <0.001 (13) | |||||||
| 16 | clr-1(e1745); epi-1(RNAi) | 48 | 0.7±0.8 | <0.001 (6) | >0.05 (13) | |||||||
| 17 | clr-1(e1745); pat-2(RNAi) | 61 | 0.7±1.0 | <0.001 (7) | >0.05 (13) | |||||||
| 18 | let-60(n1046) | Ras GTPase (gain of function) | 44 | 0.8±0.8 | – | – | ||||||
| 19 | let-60(n1046); lam-1(RNAi) | 27 | 3.1±1.0 | <0.001 (4) | <0.001 (18) | |||||||
| 20 | let-60(n1046); lam-2(RNAi) | 25 | 3.0±1.6 | <0.001 (5) | <0.001 (18) | |||||||
| 21 | let-60(n1046); epi-1(RNAi) | 60 | 2.6±1.6 | <0.001 (6) | <0.001 (18) | |||||||
| 22 | let-60(n1046); pat-2(RNAi) | 71 | 2.3±2.2 | <0.001 (7) | <0.001 (18) | |||||||
The trls10 background was used for all counts. Unless otherwise indicated, all counts used late L4 or young adult worms from mixed-stage populations maintained at 20°C
All EME counts represent the mean±s.d.
P values derived from Student's t-test with respect to the appropriate negative control (comparison row indicated in brackets)