Two-hybrid interactions
| . | Bait . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Prey . | Grk amino acids 74-245 . | Grk amino acids 179-245 . | Grk amino acids 215-245 . | CG 18501 . | |||
| Cni amino acids 2-144 | +++* | +++ | +++ | – | |||
| cni N-terminal amino acids 2-57 | n.d. | +++ | +++ | n.d. | |||
| Cnir amino acids 2-157 | n.d. | – | – | – | |||
| Staufen dsRNA BD 5 | –* | – | – | +++ | |||
| . | Bait . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Prey . | Grk amino acids 74-245 . | Grk amino acids 179-245 . | Grk amino acids 215-245 . | CG 18501 . | |||
| Cni amino acids 2-144 | +++* | +++ | +++ | – | |||
| cni N-terminal amino acids 2-57 | n.d. | +++ | +++ | n.d. | |||
| Cnir amino acids 2-157 | n.d. | – | – | – | |||
| Staufen dsRNA BD 5 | –* | – | – | +++ | |||
Grk binds to Cni but not to Cnir in a two-hybrid assay. The regions responsible for binding can be narrowed down to the 31 membrane proximal residues of the Grk extracellular domain and the first 57 amino acids of the Cni N terminus, containing the lumenal loop of the protein(Powers and Barlowe,2002).
Assayed with His Ade double selection to suppress Grk autoactivation. n.d. not done