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Table 1.

Nucleotide sequences of the primers and antisense morpholino origonucleotides MOs used in this study

Primers
Primers used for the amplification of the SSLP markers
 
 
mrck1 5′-TTAATGATGTGCTGACGCAGG-3′ 
 5′-GCACAAGTACAGTGTTGGTCG-3′ 
wz12343 5′-CATCAGCGTACACACGAACT-3′ 
 5′-CAGATATCGCCAACAGTGCA-3′ 
Primers used for the amplification of the partial cDNA to generate RNA probes
 
 
fz3a 5′-GTGTGGAGGAGCGCGTTTCTC-3′ (sense) 
 5′-TGAGGCTGTGGGTTCAATTCC-3′ (antisense) 
celsr1a 5′-GTTGGTTATTATGGGTTCACC-3′ (sense) 
 5′-TCCAAACAGTACGCGTCCTTC-3′ (antisense) 
celsr1b 5′-CAGTGATATGGATGTGCTTCG-3′ (sense) 
 5′-CTCATCTCGCTGATTGTGAGG-3′ (antisense) 
celsr2 5′-GGGTCCCCAAACTGCCTGAGG-3′ (sense) 
 5′-CACCAGCCACGCGGACACGGC-3′ (antisense) 
celsr3 5′-CAAAATGGCGGCACATGTCGG-3′ (sense) 
 5′-CTGTGGGTGGGGCAAGGGGAG-3′ (antisense) 
Primers for RT-PCR assay to show splicing defects induced by the MOs
 
 
fz3a 5′-ATGCTGACTGTATGCATGGCC-3′ 
 5′-GCCATAATCCCGTTGAACTGC-3′ 
celsr1a 5′-GATGACAACATCTGCCTAAGG-3′ 
 5′-GACTAGCAGGTTGACACACTC-3′ 
celsr1b 5′-CATTTGTGGCGTCTAATACAG-3′ 
 5′-GGCTTCTCGTACTCTCCGTCG-3′ 
celsr2 5′-TTCGTGACGTCCGACACCATC-3′ 
 5′-GAAGGTGATGAAGGTCTGCGG-3′ 
MOs
 
 
fz3a-MO 5′-CAATGTGAATTGGTTTACCTCCATG-3′ 
celsr1a-MO 5′-CATTTAGCAAACTCACCTGTGAAGT-3′ 
celsr1b-MO 5′-TAAGAGAATGACTGACCTGTAAAAT-3′ 
celsr2-MO 5′-GAGGCTCCGCCCTCACCTGTGTAGT-3′ 
Control MOs
 
 
fz3a-MO-5mis 5′-CAAaGTcAATTGcTTTACgTCgATG-3′ 
celsr1a-MO-5mis 5′-CATTTAcCAAAgTCACaTGTcAAcT-3 
celsr1b-MO-5mis 5′-TAAGAcAATcACTcACCTcTAAtAT-3′ 
celsr2-MO-5mis 5′-GAGcCTgCGCCCTCAgCTcTGTAcT-3′ 
Primers
Primers used for the amplification of the SSLP markers
 
 
mrck1 5′-TTAATGATGTGCTGACGCAGG-3′ 
 5′-GCACAAGTACAGTGTTGGTCG-3′ 
wz12343 5′-CATCAGCGTACACACGAACT-3′ 
 5′-CAGATATCGCCAACAGTGCA-3′ 
Primers used for the amplification of the partial cDNA to generate RNA probes
 
 
fz3a 5′-GTGTGGAGGAGCGCGTTTCTC-3′ (sense) 
 5′-TGAGGCTGTGGGTTCAATTCC-3′ (antisense) 
celsr1a 5′-GTTGGTTATTATGGGTTCACC-3′ (sense) 
 5′-TCCAAACAGTACGCGTCCTTC-3′ (antisense) 
celsr1b 5′-CAGTGATATGGATGTGCTTCG-3′ (sense) 
 5′-CTCATCTCGCTGATTGTGAGG-3′ (antisense) 
celsr2 5′-GGGTCCCCAAACTGCCTGAGG-3′ (sense) 
 5′-CACCAGCCACGCGGACACGGC-3′ (antisense) 
celsr3 5′-CAAAATGGCGGCACATGTCGG-3′ (sense) 
 5′-CTGTGGGTGGGGCAAGGGGAG-3′ (antisense) 
Primers for RT-PCR assay to show splicing defects induced by the MOs
 
 
fz3a 5′-ATGCTGACTGTATGCATGGCC-3′ 
 5′-GCCATAATCCCGTTGAACTGC-3′ 
celsr1a 5′-GATGACAACATCTGCCTAAGG-3′ 
 5′-GACTAGCAGGTTGACACACTC-3′ 
celsr1b 5′-CATTTGTGGCGTCTAATACAG-3′ 
 5′-GGCTTCTCGTACTCTCCGTCG-3′ 
celsr2 5′-TTCGTGACGTCCGACACCATC-3′ 
 5′-GAAGGTGATGAAGGTCTGCGG-3′ 
MOs
 
 
fz3a-MO 5′-CAATGTGAATTGGTTTACCTCCATG-3′ 
celsr1a-MO 5′-CATTTAGCAAACTCACCTGTGAAGT-3′ 
celsr1b-MO 5′-TAAGAGAATGACTGACCTGTAAAAT-3′ 
celsr2-MO 5′-GAGGCTCCGCCCTCACCTGTGTAGT-3′ 
Control MOs
 
 
fz3a-MO-5mis 5′-CAAaGTcAATTGcTTTACgTCgATG-3′ 
celsr1a-MO-5mis 5′-CATTTAcCAAAgTCACaTGTcAAcT-3 
celsr1b-MO-5mis 5′-TAAGAcAATcACTcACCTcTAAtAT-3′ 
celsr2-MO-5mis 5′-GAGcCTgCGCCCTCAgCTcTGTAcT-3′ 

Underlined text indicates exons. Lower-case letter indicate mispaired residues.

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