Rescue of vtn mutant phenotype by mRNA injection.
| . | Wild type . | Intermediate . | Mutant . |
|---|---|---|---|
| 100 ng/μl wild-type vtn RNA in m201 × m201 | 455 (92.8%) | 40 (8.0%) | 1 (0.2%) |
| 100 ng/μl wild-type san RNA in m201 × m201 | 37 (75.5%) | 0 (0%) | 12 (24.5%) |
| Mock injected in m201 × m201 | 142 (74%) | 0 (0%) | 50 (26%) |
| 100 ng/μl wild-type vtn RNA in m775 × m775 | 69 (74%) | 0 (0%) | 24 (26%) |
| 100 ng/μl wild-type san RNA in m775 × m775 | 183 (75%) | 0 (0%) | 61 (25%) |
| Mock injected in m775 × m775 | 60 (74%) | 0 (0%) | 19 (26%) |
| . | Wild type . | Intermediate . | Mutant . |
|---|---|---|---|
| 100 ng/μl wild-type vtn RNA in m201 × m201 | 455 (92.8%) | 40 (8.0%) | 1 (0.2%) |
| 100 ng/μl wild-type san RNA in m201 × m201 | 37 (75.5%) | 0 (0%) | 12 (24.5%) |
| Mock injected in m201 × m201 | 142 (74%) | 0 (0%) | 50 (26%) |
| 100 ng/μl wild-type vtn RNA in m775 × m775 | 69 (74%) | 0 (0%) | 24 (26%) |
| 100 ng/μl wild-type san RNA in m775 × m775 | 183 (75%) | 0 (0%) | 61 (25%) |
| Mock injected in m775 × m775 | 60 (74%) | 0 (0%) | 19 (26%) |
Injection of vtn mRNA, but not san mRNA, into the progeny of m201 heterozygotes is able to rescue the vtn mutant phenotype. Neither san nor vtn mRNA injection is able to rescue the san mutant phenotype.