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Table 1.

Density of INCs and CCPs during radial intercalation

CCP-apicalCCP-6 μmCCP-10 μmINC-apicalINC-6 μm
t=1
 

 

 

 

 

 
Control 28±7 39±9 41±9 23±7 42±13 
dnHMM 26±10 56±15 71±20 31±13 48±21 
t=2
 

 

 

 

 

 
Control 32±9 40±10 42±10 28±11 38±11 
dnHMM 45±18 74±14 88±17 49±23 65±23 
t=3
 

 

 

 

 

 
Control 37±4 45±5 47±6 26±9 32±11 
dnHMM 43±11 75±18 87±18 53±19 70±24 
t=4
 

 

 

 

 

 
Control 35±7 41±6 43±6 26±9 33±8 
dnHMM 42±20 66±25 76±21 54±10 72±17 
t=5
 

 

 

 

 

 
Control 32±5 37±6 37±6 19±9 29±15 
dnHMM
 
44±16
 
65±19
 
73±14
 
55±15
 
76±34
 
CCP-apicalCCP-6 μmCCP-10 μmINC-apicalINC-6 μm
t=1
 

 

 

 

 

 
Control 28±7 39±9 41±9 23±7 42±13 
dnHMM 26±10 56±15 71±20 31±13 48±21 
t=2
 

 

 

 

 

 
Control 32±9 40±10 42±10 28±11 38±11 
dnHMM 45±18 74±14 88±17 49±23 65±23 
t=3
 

 

 

 

 

 
Control 37±4 45±5 47±6 26±9 32±11 
dnHMM 43±11 75±18 87±18 53±19 70±24 
t=4
 

 

 

 

 

 
Control 35±7 41±6 43±6 26±9 33±8 
dnHMM 42±20 66±25 76±21 54±10 72±17 
t=5
 

 

 

 

 

 
Control 32±5 37±6 37±6 19±9 29±15 
dnHMM
 
44±16
 
65±19
 
73±14
 
55±15
 
76±34
 

Table shows average number of cells per 100 outer cells. The number of each cell type (CCP, INC) was determined per field based on confocal images(Fig. 5) of control, mRFP injected and dnHMM + mRFP-injected embryos. Cells were counted at the indicated time points (t=1 to t=5), representing stages 18-22. The number of each cell type was standardized to 100 outer cells, and the average of data taken from three embryos (two to four fields per embryo). A field is defined as an area 191 μm by 191 μm containing between 55 and 70 outer cells.

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