Synergistic effects of Dll1 and Jag2 mutations on hair cell numbers
| Genotype . | . | . | Counts/100 μm (mean±s.e.m.) . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dll1 . | Jag2 . | n . | IHCs . | OHCs . | HCs . | OHC/IHC . | ||||
| +/+ | +/+ | 3 | 13.6±0.6 | 44.3±2.3 | 57.9±2.6 | 3.3 | ||||
| +/− | +/+ | 3 | 13.8±0.3 | 45.5±0.6 | 58.8±0.8 | 3.3 | ||||
| +/hyp | +/+ | 3 | 13.2±0.9 | 44.1±1.6 | 57.3±2.5 | 3.3 | ||||
| +/+ | +/− | 4 | 14.2±0.4 | 44.0±1.2 | 58.2±1.6 | 3.2 | ||||
| +/hyp | +/− | 3 | 15.0±0.1 | 46.8±0.8 | 61.7±1.2 | 3.1 | ||||
| hyp/− | +/+ | 3 | 14.3±0.4 | 46.6±0.2 | 60.9±0.5 | 3.3 | ||||
| +/− | +/− | 4 | 17.4±0.5*** | 50.9±0.9* | 68.9±1.0** | 2.9 | ||||
| hyp/− | +/− | 3 | 18.9±1.0*** | 52.0±3.6* | 70.9±4.4*** | 2.8 | ||||
| +/+ | −/− | 3 | 22.8±0.7*** | 50.6±2.6* | 73.4±3.3*** | 2.2 | ||||
| Genotype . | . | . | Counts/100 μm (mean±s.e.m.) . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dll1 . | Jag2 . | n . | IHCs . | OHCs . | HCs . | OHC/IHC . | ||||
| +/+ | +/+ | 3 | 13.6±0.6 | 44.3±2.3 | 57.9±2.6 | 3.3 | ||||
| +/− | +/+ | 3 | 13.8±0.3 | 45.5±0.6 | 58.8±0.8 | 3.3 | ||||
| +/hyp | +/+ | 3 | 13.2±0.9 | 44.1±1.6 | 57.3±2.5 | 3.3 | ||||
| +/+ | +/− | 4 | 14.2±0.4 | 44.0±1.2 | 58.2±1.6 | 3.2 | ||||
| +/hyp | +/− | 3 | 15.0±0.1 | 46.8±0.8 | 61.7±1.2 | 3.1 | ||||
| hyp/− | +/+ | 3 | 14.3±0.4 | 46.6±0.2 | 60.9±0.5 | 3.3 | ||||
| +/− | +/− | 4 | 17.4±0.5*** | 50.9±0.9* | 68.9±1.0** | 2.9 | ||||
| hyp/− | +/− | 3 | 18.9±1.0*** | 52.0±3.6* | 70.9±4.4*** | 2.8 | ||||
| +/+ | −/− | 3 | 22.8±0.7*** | 50.6±2.6* | 73.4±3.3*** | 2.2 | ||||
Inner hair cells (IHCs) and outer hair cells (OHCs) were counted separately for each indicated genotype, and were then combined to get total numbers of hair cells (HCs). Counts were expressed in numbers of hair cells per 100μm, to take into account the slightly different lengths that were counted for each cochlea. Counts were also expressed as outer hair cell versus inner hair cell ratios (OHC/IHC). Significant increases in hair cells numbers were not observed until more than one copy of a Notch ligand (Dll1 or Jag2) was deleted or reduced. Significance was determined by comparing each of the mutant groups with the control group (+/+; +/+), using Dunnett's method.
*P<0.05; **P<0.01; ***P<0.0001.