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Table 1.

Quantitative analyses of marker gene expression

	Targets
Injected	Gli1	Gli2	Gli3	Ptc1	Ptc2	Ncam	Ngn1a	NeuroD	Ntub
Stage 12
Gli1+MO-C		10.1^*	0.5^*	36.5^*	135.2^*	0.6^*	1.5^*	1.0^*	14.0^*
Gli1+MO-2			0.7	0.8	0.6	0.8	1.0	1.1	0.9
Gli1+MO-3		1.1		0.5	0.3	1.3	1.1	0.7	0.4
Gli2+MO-C	6.3^*		0.5^*	12.2^*	29.2^*	1.0^*	1.6^*	0.5^*	19.8^*
Gli2+MO-1			0.7	0.7	0.6	0.6	0.6	0.3	0.5
Gli2+MO-3	0.4			0.5	0.6	2.1	1.4	1.0	0.4
Gli3+MO-C	0.9^*	13.2^*		10.3^*	18.2^*	1.4^*	4.8^*	2.7^*	4.6^*
Gli3+MO-1		0.5		0.9	0.6	1.0	0.4	0.2	0.7
Gli3+MO-2	0.8			0.5	0.6	0.7	1.2	0.6	0.3
Stage 14
Gli1+MO-C		21.0^*	1.0^*	39.5^*	84.5^*	0.4^*	7.5^*	7.9^*	4.7^*
Gli1+MO-2			0.7	1.0	0.7	0.9	0.8	0.7	0.7
Gli1+MO-3		0.9		0.8	0.8	1.3	1.8	0.7	0.5
Gli2+MO-C	20.1^*		2.7^*	10.4^*	23.0^*	1.3^*	15.1^*	1.4^*	28.8^*
Gli2+MO-1			0.4	0.5	0.7	0.6	0.6	1.5	0.4
Gli2+MO-3	0.3			0.3	0.2	0.6	0.6	2.8	0.1
Gli3+MO-C	6.3^*	28.4^*		10.6^*	12.5^*	2.0^*	16.3^*	3.7^*	8.4^*
Gli3+MO-1		0.4		0.5	0.6	0.5	0.4	0.8	0.2
Gli3+MO-2	0.4			0.5	1.2	0.7	0.4	0.7	0.1
Stage 14
GLI1+MO-C	6.9^*	5.2^*	1.3^*	20.9^*	64.5^*	1.2^*	2.6^*	0.5^*	2.6^*
GLI1+MO-1		0.8	3.5	0.1	0.2	2.0	0.5	1.9	0.7
GLI1+MO-2	1.1		1.3	0.9	0.8	5.6	2.5	4.0	1.6
GLI1+MO-3	0.7	0.7		>0	>0	2.4	1.1	3.3	0.2
GLI2+MO-C	19.7^*	5.5^*	2.0^*	18.3^*	17.4^*	6.3^*	4.3^*	16.5^*	22.8^*
GLI2+MO-1		0.9	1.2	0.5	0.8	0.4	1.2	>0	0.3
GLI2+MO-2	1.2		1.9	2.2	1.0	0.4	1.4	>0	0.2
GLI2+MO-3	1.0	1.5		0.8	1.2	0.9	5.2	2.0	0.3
GLI3+MO-C	9.5^*	22.2^*	3.7^*	10.6^*	4.9^*	4.1^*	15.1^*	4.6^*	34.8^*
GLI3+MO-1		0.3	0.3	0.4	1.3	1.2	0.5	0.1	0.2
GLI3+MO-2	0.9		0.3	0.7	1.4	2.4	0.5	0.2	0.7
GLI3+MO-3	1.0	0.8		0.9	1.8	0.4	0.4	0.8	0.7

	Targets
Injected	Gli1	Gli2	Gli3	Ptc1	Ptc2	Ncam	Ngn1a	NeuroD	Ntub
Stage 12
Gli1+MO-C		10.1^*	0.5^*	36.5^*	135.2^*	0.6^*	1.5^*	1.0^*	14.0^*
Gli1+MO-2			0.7	0.8	0.6	0.8	1.0	1.1	0.9
Gli1+MO-3		1.1		0.5	0.3	1.3	1.1	0.7	0.4
Gli2+MO-C	6.3^*		0.5^*	12.2^*	29.2^*	1.0^*	1.6^*	0.5^*	19.8^*
Gli2+MO-1			0.7	0.7	0.6	0.6	0.6	0.3	0.5
Gli2+MO-3	0.4			0.5	0.6	2.1	1.4	1.0	0.4
Gli3+MO-C	0.9^*	13.2^*		10.3^*	18.2^*	1.4^*	4.8^*	2.7^*	4.6^*
Gli3+MO-1		0.5		0.9	0.6	1.0	0.4	0.2	0.7
Gli3+MO-2	0.8			0.5	0.6	0.7	1.2	0.6	0.3
Stage 14
Gli1+MO-C		21.0^*	1.0^*	39.5^*	84.5^*	0.4^*	7.5^*	7.9^*	4.7^*
Gli1+MO-2			0.7	1.0	0.7	0.9	0.8	0.7	0.7
Gli1+MO-3		0.9		0.8	0.8	1.3	1.8	0.7	0.5
Gli2+MO-C	20.1^*		2.7^*	10.4^*	23.0^*	1.3^*	15.1^*	1.4^*	28.8^*
Gli2+MO-1			0.4	0.5	0.7	0.6	0.6	1.5	0.4
Gli2+MO-3	0.3			0.3	0.2	0.6	0.6	2.8	0.1
Gli3+MO-C	6.3^*	28.4^*		10.6^*	12.5^*	2.0^*	16.3^*	3.7^*	8.4^*
Gli3+MO-1		0.4		0.5	0.6	0.5	0.4	0.8	0.2
Gli3+MO-2	0.4			0.5	1.2	0.7	0.4	0.7	0.1
Stage 14
GLI1+MO-C	6.9^*	5.2^*	1.3^*	20.9^*	64.5^*	1.2^*	2.6^*	0.5^*	2.6^*
GLI1+MO-1		0.8	3.5	0.1	0.2	2.0	0.5	1.9	0.7
GLI1+MO-2	1.1		1.3	0.9	0.8	5.6	2.5	4.0	1.6
GLI1+MO-3	0.7	0.7		>0	>0	2.4	1.1	3.3	0.2
GLI2+MO-C	19.7^*	5.5^*	2.0^*	18.3^*	17.4^*	6.3^*	4.3^*	16.5^*	22.8^*
GLI2+MO-1		0.9	1.2	0.5	0.8	0.4	1.2	>0	0.3
GLI2+MO-2	1.2		1.9	2.2	1.0	0.4	1.4	>0	0.2
GLI2+MO-3	1.0	1.5		0.8	1.2	0.9	5.2	2.0	0.3
GLI3+MO-C	9.5^*	22.2^*	3.7^*	10.6^*	4.9^*	4.1^*	15.1^*	4.6^*	34.8^*
GLI3+MO-1		0.3	0.3	0.4	1.3	1.2	0.5	0.1	0.2
GLI3+MO-2	0.9		0.3	0.7	1.4	2.4	0.5	0.2	0.7
GLI3+MO-3	1.0	0.8		0.9	1.8	0.4	0.4	0.8	0.7

Numbers indicate fold change in endogenous gene expression in stage 12 or 14 animal caps with frog Gli or human GLI RNAs and MOs, determined by real-time RT-PCR analyses as calculated by the ΔCT method. Gene expression levels were normalized to EF1α. Changes of at least 40% are in green for activator function and in red for repressor function.

MO-C=Gli`x'+MO-C/uninjected AC.

MO-`x'=Gli`x'+MO-`y'/Gli`x'+MO-C.

The action of injected Gli proteins.

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