IsO expression of lmx1b.1 and lmx1b.2 in offspring of no isthmus heterozygotes
| . | Lmx1b.1 . | . | . | Lmx1b.2 . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Stage . | Normal . | Absent . | % absent . | Normal . | Absent . | % absent . | ||||
| One somite | 17 | 0 | 0.0 | 17 | 0 | 0.0 | ||||
| Five somites | 52 | 10 | 16.1 | 33 | 10 | 23.3 | ||||
| Seven somites | 30 | 15 | 33.3 | 33 | 17 | 34.0 | ||||
| Twelve somites | 63 | 24 | 27.6 | 69 | 28 | 28.9 | ||||
| Eighteen somites | 30 | 10 | 25.0 | 15 | 9 | 37.5 | ||||
| . | Lmx1b.1 . | . | . | Lmx1b.2 . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Stage . | Normal . | Absent . | % absent . | Normal . | Absent . | % absent . | ||||
| One somite | 17 | 0 | 0.0 | 17 | 0 | 0.0 | ||||
| Five somites | 52 | 10 | 16.1 | 33 | 10 | 23.3 | ||||
| Seven somites | 30 | 15 | 33.3 | 33 | 17 | 34.0 | ||||
| Twelve somites | 63 | 24 | 27.6 | 69 | 28 | 28.9 | ||||
| Eighteen somites | 30 | 10 | 25.0 | 15 | 9 | 37.5 | ||||
Heterozygous parents were crossed and offspring were fixed at several different stages and examined for expression of lmx1b.1 or lmx1b.2. Absence of expression in 25% of the embryos corresponds to the predicted frequency of no isthmus homozygous offspring.