pro-1(na48) genetic interactions and RNAi phenotypes
| (A) . | Genotype*,† . | % Pro . | % Pex . | n . |
|---|---|---|---|---|
| pro-1(na48) | 66 | 0 | 92 | |
| mpk-1(ga111) | 0 | 91 | 162 | |
| pro-1(na48);mpk-1(ga111) | 64‡ | 100 | 98 |
| (A) . | Genotype*,† . | % Pro . | % Pex . | n . |
|---|---|---|---|---|
| pro-1(na48) | 66 | 0 | 92 | |
| mpk-1(ga111) | 0 | 91 | 162 | |
| pro-1(na48);mpk-1(ga111) | 64‡ | 100 | 98 |
| (B) . | Genotype*,§,¶ . | % Pro . | % Fem . | n . |
|---|---|---|---|---|
| pro-1(na48) | 58** | 0 | 160 | |
| fem-1(hc17) | 0 | 100 | 152 | |
| pro-1(na48); fem-1(hc17) | 43 | 90†† | 171 |
| (B) . | Genotype*,§,¶ . | % Pro . | % Fem . | n . |
|---|---|---|---|---|
| pro-1(na48) | 58** | 0 | 160 | |
| fem-1(hc17) | 0 | 100 | 152 | |
| pro-1(na48); fem-1(hc17) | 43 | 90†† | 171 |
| (C) . | Genotype*,§,‡‡ . | Hermaphrodite % Pro(n) . | Male % Pro(n) . | . |
|---|---|---|---|---|
| pro-1(na48); him-5(e1490) | 74(80)§§ | 23 (40) |
| (C) . | Genotype*,§,‡‡ . | Hermaphrodite % Pro(n) . | Male % Pro(n) . | . |
|---|---|---|---|---|
| pro-1(na48); him-5(e1490) | 74(80)§§ | 23 (40) |
| (D) . | Genotype*,§,¶¶ . | % Pro . | % Glp . | n . |
|---|---|---|---|---|
| pro-1(na48) | 84 | 0 | 62 | |
| glp-1(e2141) | 0 | 100 | 62 | |
| pro-1(na48);glp-1(e2141) | 0 | 100 | 88 |
| (D) . | Genotype*,§,¶¶ . | % Pro . | % Glp . | n . |
|---|---|---|---|---|
| pro-1(na48) | 84 | 0 | 62 | |
| glp-1(e2141) | 0 | 100 | 62 | |
| pro-1(na48);glp-1(e2141) | 0 | 100 | 88 |
| (E) . | Genotype*,§,*** . | % Pro . | % Vul . | n . |
|---|---|---|---|---|
| pro-1(na48) | 75** | 0 | 60 | |
| lin-12(n302) | 0 | 98 | 522 | |
| pro-1(na48);lin-12(n302) | 68 | 100 | 72 |
| (E) . | Genotype*,§,*** . | % Pro . | % Vul . | n . |
|---|---|---|---|---|
| pro-1(na48) | 75** | 0 | 60 | |
| lin-12(n302) | 0 | 98 | 522 | |
| pro-1(na48);lin-12(n302) | 68 | 100 | 72 |
| (F) . | Genotype . | %Gon††† . | n . | . |
|---|---|---|---|---|
| pro-1(RNAi) | 100 | 122 | ||
| rrf-1(pk1417); pro-1(RNAi) | 6 | 296 |
| (F) . | Genotype . | %Gon††† . | n . | . |
|---|---|---|---|---|
| pro-1(RNAi) | 100 | 122 | ||
| rrf-1(pk1417); pro-1(RNAi) | 6 | 296 |
| (G) . | Genotype*,‡‡‡ . | % Pro at 20°C . | % Glp at 20°C . | n . |
|---|---|---|---|---|
| pro-1(na48) | 11 | 0 | 82 | |
| glp-1(e2141) | 0 | 4 | 212 | |
| pro-1(na48);glp-1(e2141) | 0 | 31 | 58 |
| (G) . | Genotype*,‡‡‡ . | % Pro at 20°C . | % Glp at 20°C . | n . |
|---|---|---|---|---|
| pro-1(na48) | 11 | 0 | 82 | |
| glp-1(e2141) | 0 | 4 | 212 | |
| pro-1(na48);glp-1(e2141) | 0 | 31 | 58 |
| (H) . | Genotype*,§,§§§ . | % Pro at 20°C . | n . | . |
|---|---|---|---|---|
| pro-1(na48) | 19 | 208 | ||
| glp-1(ar202) | 0 | 152 | ||
| pro-1(na48);glp-1(ar202) | 72 | 118 |
| (H) . | Genotype*,§,§§§ . | % Pro at 20°C . | n . | . |
|---|---|---|---|---|
| pro-1(na48) | 19 | 208 | ||
| glp-1(ar202) | 0 | 152 | ||
| pro-1(na48);glp-1(ar202) | 72 | 118 |
n, number of gonad arms; Vul, number of animals scored equals half n.
Homozygous pro-1(na48): self progeny of heterozygous parents balanced by mIn1.
Strains marked with unc-79(e1068), grown at 20°C and shifted to 25°C as gravid adults. L1 progeny were synchronized and cultured at 25°C for 72 hours [pro-1(na48)-containing strains] or 48 hours,before fixation and DAPI staining. Pex (pachytene exit defect): mitotic,transition and pachytene nuclei present but no gametes.
Pro here is a Pro/Pex phenotype (see text).
Strains marked with unc-4(e120).
Strains grown at 20°C. Synchronized L1 animals were shifted to 25°C and scored after 72 hours (pro-1(na48)-containing strains) or 48 hours.
These values are not significantly different (P≫0.05) from the corresponding values two rows directly below (two-sided Fisher exact test).
Remaining 10% non-Fem animals were non-Pro, non-Fem.
Animals fixed and DAPI stained after 72 hours.
This value is significantly different from the value in the next column(P<2×10−7, based on two-sided Fisher exact test).
Strains marked with dpy-17(e164) and grown at 20°C. Synchronized L1 animals were shifted to 25°C.
For pro-1(na48)-containing strains, mid-L4s were picked to a fresh plate and fixed and DAPI stained 24 hours later. For unc-4(e120);lin-12(n302), all adults from several mixed-stage plates were scored under Normarki optics for Vul and Pro phenotypes.
Asynchronous adults scored for the Gon phenotype under Nomarski optics.
Strains homozygous for him-5(e1490), grown at 20°C,synchronized, fixed and DAPI stained after 72 hours for glp-1(e2141);him-5(e1490), and after 96 hours for pro-1(na48)-containing strains.
Animals grown at 20°C, synchronized, fixed and DAPI-stained after 72 hours for unc-4(e120); glp-1(ar202), and 96 hours for pro-1(na48)-containing strains.