Genetic dose and temperature dependence of pro-1 mutant phenotypes
| Genotype . | Temperature (°C) . | Pro* (%) . | Mig† (%) . | Gametogenesis‡(%) . | Abnormal vulva§ (%) . | n¶ . | Hours to adult molt** . | Relative rate of development†† . |
|---|---|---|---|---|---|---|---|---|
| pro-1 (+) | 25 | 0 | 1 | 100 | 0 | 180 | 35.5 | 1 |
| pro-1(+)/pro-1(na48) | 25 | 0 | 1 | 100 | 0 | 112 | 35.5 | 1 |
| pro-1(na48) | 25 | 83 | 38 | 100 | 0 | 138 | 54 | 0.66 |
| pro-1(na48)/Df‡‡ | 25 | 57 | 69 | 100 | 0 | 54 | 85 | 0.42 |
| pro-1(RNAi)§§ | 25 | 1 | 53 | 73 | Pvl 17 | 122 | ND | ND |
| pro-1(+) | 20 | 0 | 1 | 100 | 0 | 108 | 47.5 | 1 |
| pro-1(+)/pro-1(na48) | 20 | 0 | 1 | 100 | 0 | 126 | 47.5 | 1 |
| pro-1(na48)¶¶ | 20 | 11 | 56 | 100 | 0 | 130 | 78 | 0.61 |
| pro-1(na48)/Df*** | 20 | 20 | 80 | 100 | Vul 7, Pvl 21 | 56 | ∼110 | 0.43 |
| pro-1(+) | 15 | 0 | 0 | 100 | 0 | 122 | 78 | 1 |
| pro-1(+)/pro-1(na48) | 15 | 0 | 0 | 100 | 0 | 118 | 78 | 1 |
| pro-1(na48) | 15 | 0 | 65 | 66 | Pvl 9 | 114 | ∼168 | 0.46 |
| pro-1(na48)/Df | 15 | 0 | 100 | 36 | Vul 100 | 28 | ∼240 | 0.33 |
| Genotype . | Temperature (°C) . | Pro* (%) . | Mig† (%) . | Gametogenesis‡(%) . | Abnormal vulva§ (%) . | n¶ . | Hours to adult molt** . | Relative rate of development†† . |
|---|---|---|---|---|---|---|---|---|
| pro-1 (+) | 25 | 0 | 1 | 100 | 0 | 180 | 35.5 | 1 |
| pro-1(+)/pro-1(na48) | 25 | 0 | 1 | 100 | 0 | 112 | 35.5 | 1 |
| pro-1(na48) | 25 | 83 | 38 | 100 | 0 | 138 | 54 | 0.66 |
| pro-1(na48)/Df‡‡ | 25 | 57 | 69 | 100 | 0 | 54 | 85 | 0.42 |
| pro-1(RNAi)§§ | 25 | 1 | 53 | 73 | Pvl 17 | 122 | ND | ND |
| pro-1(+) | 20 | 0 | 1 | 100 | 0 | 108 | 47.5 | 1 |
| pro-1(+)/pro-1(na48) | 20 | 0 | 1 | 100 | 0 | 126 | 47.5 | 1 |
| pro-1(na48)¶¶ | 20 | 11 | 56 | 100 | 0 | 130 | 78 | 0.61 |
| pro-1(na48)/Df*** | 20 | 20 | 80 | 100 | Vul 7, Pvl 21 | 56 | ∼110 | 0.43 |
| pro-1(+) | 15 | 0 | 0 | 100 | 0 | 122 | 78 | 1 |
| pro-1(+)/pro-1(na48) | 15 | 0 | 0 | 100 | 0 | 118 | 78 | 1 |
| pro-1(na48) | 15 | 0 | 65 | 66 | Pvl 9 | 114 | ∼168 | 0.46 |
| pro-1(na48)/Df | 15 | 0 | 100 | 36 | Vul 100 | 28 | ∼240 | 0.33 |
ND, not determined.
Mitotic germ cells proximal to gametes. In a separate experiment, 72 hours post-hatch-off, 63% of Pro arms (n=94; 72% Pro) contained both oocytes and sperm and remainder contained only sperm. At 96 hours, 84% of Pro arms (n=70; 71% Pro) contained both oocytes and sperm. Therefore,reduced oogenesis may reflect a delay in the spermatogenesis/oogenesis switch.
Abnormal DTC migration. Defects seen in dorsal, centrifugal or both migrations. Mig and Pro did not correlate nor were they mutually exclusive.
Spermatocytes, sperm and/or oocyte nuclei observed with DAPI or Nomarski.
Vul, Vulvaless; Pvl, protruding vulva (versus Evl, abnormally everted vulva).
n=number of gonad arms scored. For vulva phenotypes, the number of animals scored is n/2.
Hours to adult molt from the hatch-off (see Materials and methods).
Relative development rate: hours from hatch-off to adult molt in wild-type over mutant; ratio gives comparative rates of development at different temperatures.
Df = mnDf58. pro-1 (na48)/mnDf58 were synchronized non-GFP progeny of pro-1(na48)/mInl males crossed to mnDf58/mInlhermaphrodites. Pro data alone are inconsistent with pro-1(na48) as a simple reduction of function allele. This inconsistency may result from temperature-sensitive haploinsufficiency of another locus under the deficiency.
Data are reported from a single experiment (see Materials and methods);similar results were obtained from multiple RNAi experiments.
Although pro-1 (na48) is only 11% Pro at 20°C, the strain is difficult to maintain because of Emo sterility. Emo: 33%(29/86) of gonad arms,scored by DAPI at 120 hours post-hatch-off.
One individual Vul pro-1(na48)/mnDf58 animal displayed the Pro phenotype in both gonad arms.