OLIG1 and OLIG2 directly bind to ID2 and ID4
| . | Number of colonies . | . | . | ||
|---|---|---|---|---|---|
| Constructs . | pBT-OLIG+ pTRG-ID (test) . | pBT-OLIG+ pTRG (negative control) . | pBT + pTRG-ID (negative control) . | ||
| OLIG1 + ID2 | 79 | 5 | 3 | ||
| OLIG1 +ID4 | 60 | 2 | 0 | ||
| OLIG2 + ID2 | 97 | 4 | 5 | ||
| OLIG2 + ID4 | 83 | 1 | 6 | ||
| . | Number of colonies . | . | . | ||
|---|---|---|---|---|---|
| Constructs . | pBT-OLIG+ pTRG-ID (test) . | pBT-OLIG+ pTRG (negative control) . | pBT + pTRG-ID (negative control) . | ||
| OLIG1 + ID2 | 79 | 5 | 3 | ||
| OLIG1 +ID4 | 60 | 2 | 0 | ||
| OLIG2 + ID2 | 97 | 4 | 5 | ||
| OLIG2 + ID4 | 83 | 1 | 6 | ||
Bacterial two-hybrid analyses was carried out to detect possible direct binding using bait vectors pBT-OLIG1 or pBT-OLIG2 and target vectors pTRG-ID2 or pTRG-ID4. The negligible number of colonies in the negative control plates and the large number of colonies in the test plates indicate a positive interaction of OLIG proteins with ID2 and ID4. No interactions were detected with ID1 or ID3 (not shown).