Quantification of cell proliferation in the hindbrain of hdac1hi1618 mutant and unaffected sibling embryos at 25,33 and 38 hpf
.
Age
.
.
.
.
25 hpf
.
33 hpf
.
38 hpf
.
hdac1hi1618 siblings
285±11.5
306±2.9
300±17.8
hdac1hi1618 mutants
176±14.6
320±14.3
288±8.6
.
Age
.
.
.
.
25 hpf
.
33 hpf
.
38 hpf
.
hdac1hi1618 siblings
285±11.5
306±2.9
300±17.8
hdac1hi1618 mutants
176±14.6
320±14.3
288±8.6
The total number of darkly stained phospho-H3-positive nuclei in the region of the hindbrain spanning rhombomeres 2-7 was counted under the compound microscope. Values given are mean cell counts (±s.e.m.) where n=4 individual embryos in each sample group (see Fig. 3 for examples).