her3 deletion constructs and effects of injecting mRNA
| Construct (300 ng) . | Σ . | Wild-type phenotype . | Normal neurog1 expression and enlargement of the neural plate (%) . | Reduced neurog1 expression and enlargement of the neural plate (%) . |
|---|---|---|---|---|
| her3 | 59 (100%) | 19% | 61% | 20% |
| her3 (600 ng) | 63 (100%) | 5% | 44% | 51% |
| her3 Δ N | 62 (100%) | 42% | 48% | 10% |
| her3 Δ b | 60 (100%) | 13% | 80% | 7% |
| her3 Δ HLH | 65 (100%) | 17% | 71% | 12% |
| her3 Δ bHLH | 62 (100%) | 18% | 69% | 13% |
| her3 Δ bHLH (600 ng) | 108 (100%) | 83% | 14% | 2% |
| her3 Δ orange | 61 (100%) | 11% | 20% | 69% |
| her3 Δ C | 63 (100%) | 13% | 16% | 71% |
| her3 Δ WRPW | 64 (100%) | 50% | 44% | 6% |
| her3 Δ WRPW (600 ng) | 90 (100%) | 89% | 11% | – |
| Construct (300 ng) . | Σ . | Wild-type phenotype . | Normal neurog1 expression and enlargement of the neural plate (%) . | Reduced neurog1 expression and enlargement of the neural plate (%) . |
|---|---|---|---|---|
| her3 | 59 (100%) | 19% | 61% | 20% |
| her3 (600 ng) | 63 (100%) | 5% | 44% | 51% |
| her3 Δ N | 62 (100%) | 42% | 48% | 10% |
| her3 Δ b | 60 (100%) | 13% | 80% | 7% |
| her3 Δ HLH | 65 (100%) | 17% | 71% | 12% |
| her3 Δ bHLH | 62 (100%) | 18% | 69% | 13% |
| her3 Δ bHLH (600 ng) | 108 (100%) | 83% | 14% | 2% |
| her3 Δ orange | 61 (100%) | 11% | 20% | 69% |
| her3 Δ C | 63 (100%) | 13% | 16% | 71% |
| her3 Δ WRPW | 64 (100%) | 50% | 44% | 6% |
| her3 Δ WRPW (600 ng) | 90 (100%) | 89% | 11% | – |