Ten gametophytic maternal effect mutants were isolated from a KS117-based screen.
| . | Reciprocal crosses: % mutant phenotypes* . | . | Genetic mapping . | |
|---|---|---|---|---|
| Line no. . | Mutant female × WT male (n) . | WT female × mutant male (n) . | Closest marker(s)† . | |
| JF1348 (dme-4) | 43.8±5.3 (164) | 0 (254) | CER479331 (0/138) | |
| JF0531 (mea-5) | 45.6±7.2 (274) | 0 (263) | nT7i23 (0/44) | |
| JF1760 (mea-6) | 34.4±9.0 (103) | 0 (218) | nT7i23 (0/66) | |
| JF3206 (mea-7) | 43.5±10.9 (123) | 0 (110) | nT7i23 (0/66) | |
| JF2034 (fis2-6) | 47.7±2.7 (122) | 0 (115) | Fis2sslp (0/68) | |
| JF2206 (fis2-7) | 39.0±7.3 (168) | 0 (187) | Fis2sslp (0/70) | |
| JF0122 (fie-10) | 55.4±5.8 (74) | 0 (148) | CER456484 (0/88) | |
| JF1762 (fie-11) | 47.1±15.8 (153) | 0 (153) | CER456484 (0/88) | |
| JF1728 (bga-1) | 28.2±13.0 (301) | 0 (238) | between PLS7 (7/142) and nga1126 (6/142) | |
| JF2973 (medicis, msi1-2) | 52.1±10.0 (691) | 0 (295) | between MT120.1/2 (2/736) and MZN1.1/2 (2/736) | |
| . | Reciprocal crosses: % mutant phenotypes* . | . | Genetic mapping . | |
|---|---|---|---|---|
| Line no. . | Mutant female × WT male (n) . | WT female × mutant male (n) . | Closest marker(s)† . | |
| JF1348 (dme-4) | 43.8±5.3 (164) | 0 (254) | CER479331 (0/138) | |
| JF0531 (mea-5) | 45.6±7.2 (274) | 0 (263) | nT7i23 (0/44) | |
| JF1760 (mea-6) | 34.4±9.0 (103) | 0 (218) | nT7i23 (0/66) | |
| JF3206 (mea-7) | 43.5±10.9 (123) | 0 (110) | nT7i23 (0/66) | |
| JF2034 (fis2-6) | 47.7±2.7 (122) | 0 (115) | Fis2sslp (0/68) | |
| JF2206 (fis2-7) | 39.0±7.3 (168) | 0 (187) | Fis2sslp (0/70) | |
| JF0122 (fie-10) | 55.4±5.8 (74) | 0 (148) | CER456484 (0/88) | |
| JF1762 (fie-11) | 47.1±15.8 (153) | 0 (153) | CER456484 (0/88) | |
| JF1728 (bga-1) | 28.2±13.0 (301) | 0 (238) | between PLS7 (7/142) and nga1126 (6/142) | |
| JF2973 (medicis, msi1-2) | 52.1±10.0 (691) | 0 (295) | between MT120.1/2 (2/736) and MZN1.1/2 (2/736) | |
n, Number of seeds. For each mutant, genetic data (reciprocal crosses between a heterozygote and a WT plant) show that the mutant phenotype is under gametophytic maternal control: a mutant maternal allele is sufficient to cause seed abortion, even when using wild-type pollen, whereas mutant pollen does not lead to abortion in any seed. Genetic mapping allowed us to determine which loci are affected in these lines. Tight linkage was found for one line with marker CER479331, for three lines with marker nT7i23, for two lines with marker fis2sslp, and for 2 lines with marker CER456484, suggesting that out of the 10 lines, 8 were allelic to one of the known fismutants and two mutants had identified new loci, borgia(bga) and medicis, with a fis-like KS117 GFP expression phenotype.
Values are means±s.d.
Number of recombinant chromosomes/total chromosome number