myod (12-14hpf) | ptc1 (12-14hpf) | nk2.2 | ptc1 | ||||||||||||
Injected mRNA | Up | igu | Up | igu | Up | igu | Up | igu | |||||||
shh (250 pg) | 15 | 5 (25%) | 16 | 4 (20%) | 20 | 4 (17%) | 20 | 10 (33%) | |||||||
dnPKA (250 pg) | 26 | 9 (26%) | 29 | 10 (26%) | 60 | 21 (26%) | 52 | 19 (27%) | |||||||
myod (12-14hpf) | ptc1 (12-14hpf) | nk2.2 | ptc1 | en1 | |||||||||||
Inhibitors | Down | igu | Down | igu | Down | igu | Down | igu | Down | igu | |||||
Cyclopamine (50 μM) | 45 | 11 (20%) | 33 | 10 (30%) | 52 | 15 (22%) | 62 | 22 (26%) | 43 | 18 (30%) | |||||
Forskolin (300 μM) | 45 | 0 (0%) | ND | 64 | 0 (0%) | 31 | 8 (20%) | 66 | 1 (2%) | ||||||
(150 μM) | 28 | 1 (3%) | ND | 45 | 8 (15%) | 38 | 13 (25%) | 44 | 5 (10%) | ||||||
(75 μM) | 18 | 6 (25%) | ND | 19 | 8 (30%) | 19 | 8 (30%) | 22 | 7 (24%) |
myod (12-14hpf) | ptc1 (12-14hpf) | nk2.2 | ptc1 | ||||||||||||
Injected mRNA | Up | igu | Up | igu | Up | igu | Up | igu | |||||||
shh (250 pg) | 15 | 5 (25%) | 16 | 4 (20%) | 20 | 4 (17%) | 20 | 10 (33%) | |||||||
dnPKA (250 pg) | 26 | 9 (26%) | 29 | 10 (26%) | 60 | 21 (26%) | 52 | 19 (27%) | |||||||
myod (12-14hpf) | ptc1 (12-14hpf) | nk2.2 | ptc1 | en1 | |||||||||||
Inhibitors | Down | igu | Down | igu | Down | igu | Down | igu | Down | igu | |||||
Cyclopamine (50 μM) | 45 | 11 (20%) | 33 | 10 (30%) | 52 | 15 (22%) | 62 | 22 (26%) | 43 | 18 (30%) | |||||
Forskolin (300 μM) | 45 | 0 (0%) | ND | 64 | 0 (0%) | 31 | 8 (20%) | 66 | 1 (2%) | ||||||
(150 μM) | 28 | 1 (3%) | ND | 45 | 8 (15%) | 38 | 13 (25%) | 44 | 5 (10%) | ||||||
(75 μM) | 18 | 6 (25%) | ND | 19 | 8 (30%) | 19 | 8 (30%) | 22 | 7 (24%) |
Embryos were obtained from igu/+ incrosses. Expected genotypes were 25% igu-/-, 50% igu-/+ and 25%homozygous wild type.
Up, respective Hh target genes are ectopically expressed. These embryos have +/+ or +/igu genotypes.
Down, Gene expression is downregulated.
igu, Hh target genes are expressed in the same pattern in igu mutants.
Percentages in parenthesis represent the ratios of embryos with igu-type gene expression to the total embryos tested for respective gene expressions. All of these embryos have igu/igu genotype. Note that igu embryos have a seemingly normal myod expression pattern.