. | Morpholino amount (ng) . | . | . | . | |||
---|---|---|---|---|---|---|---|
Morpholino . | 0 . | 1 . | 5 . | 10 . | |||
gfra1a | 100±6.3 | 97.6±5.1 | 63.5±3.9* | 60.8±3.8* | |||
gfra1b | 100±6.6 | 99.5±4.8 | 98.1±4.7 | 99.5±4.7 | |||
gfra2 | 100±6.1 | 99.2±4.3 | 100±4.4 | 96.2±4.3 | |||
ret | 100±2.9 | 92.3±2.1 | N.D. | 1.8±0.6* |
. | Morpholino amount (ng) . | . | . | . | |||
---|---|---|---|---|---|---|---|
Morpholino . | 0 . | 1 . | 5 . | 10 . | |||
gfra1a | 100±6.3 | 97.6±5.1 | 63.5±3.9* | 60.8±3.8* | |||
gfra1b | 100±6.6 | 99.5±4.8 | 98.1±4.7 | 99.5±4.7 | |||
gfra2 | 100±6.1 | 99.2±4.3 | 100±4.4 | 96.2±4.3 | |||
ret | 100±2.9 | 92.3±2.1 | N.D. | 1.8±0.6* |
Morpholino . | 0 . | 1 . | 2 . | 5 . |
---|---|---|---|---|
gfra1a+gfra1b | 100±4.8 | 95±3.4 | 6.9±0.7* | 4.7±0.5* |
Morpholino . | 0 . | 1 . | 2 . | 5 . |
---|---|---|---|---|
gfra1a+gfra1b | 100±4.8 | 95±3.4 | 6.9±0.7* | 4.7±0.5* |
Embryos were injected with morpholino oligonucleotides, fixed and stained with anti-Hu antibody at 96 hpf. For injection of gfra1a+gfra1b morpholinos, the amount of each oligonucleotide is shown. Total neurons over a 10-segment length were counted. Numbers represent percent of control±s.e.m. for at least five separate embryos per condition. *Significantly different from control(P<0.0005).
N.D., not determined.