Double mutants reveal germline defects in mat-1 mutants
| . | mat-1(ax227) 15°C . | mat-1(ax227) 25°C . | mat-1(ax212) 15°C . |
|---|---|---|---|
| mat-2(or170) apc-1* | Mel | Mel | Fertile |
| mat-3(ax148) cdc-23/apc-8* | Mel | Sterile | Fertile |
| mat-3(or180) cdc-23/apc-8* | Mel | Sterile | Mel; Sterile |
| emb-27(g48) cdc-16/apc-6† | Fertile | Mel | Fertile |
| emb-30(g53) apc-4‡ | Mel | Sterile | Mel |
| . | mat-1(ax227) 15°C . | mat-1(ax227) 25°C . | mat-1(ax212) 15°C . |
|---|---|---|---|
| mat-2(or170) apc-1* | Mel | Mel | Fertile |
| mat-3(ax148) cdc-23/apc-8* | Mel | Sterile | Fertile |
| mat-3(or180) cdc-23/apc-8* | Mel | Sterile | Mel; Sterile |
| emb-27(g48) cdc-16/apc-6† | Fertile | Mel | Fertile |
| emb-30(g53) apc-4‡ | Mel | Sterile | Mel |
Double mutants were constructed as described in the Materials and Methods. In 25°C analysis, candidate mat-1/+; mat-x/+ ormat-1/+; emb-x/+ animals were shifted from 15°C to 25°C as either embryos or L1 larvae. Mel, maternal-effect embryonic lethality in which homozygous mothers from heterozygous parents produce clutches of dead embryos.
For molecular cloning results see*Davis et al., 2002,†Golden et al.,2000,‡Furuta et al.,2000.