Genotype . | Temperature (°C) . | MI/MII . | %MI . | In utero embryo phenotype . |
---|---|---|---|---|
N2 | 20° | 11/9 | 55% | MC |
him-8 | 20° | 13/8 | 62% | MC |
ax212 | 20° | 14/9 | 61% | MC |
ax212 | 23° | 13/6 | 68% | MC |
ax212 | 24° | 24/7 | 77% | MC |
ax212 | 25° | 20/2 | 91% | MC (1PB) |
ye121 | 20° | 23/14 | 62% | MC |
ye121 | 23° | 45/16 | 74% | MC |
ye121 | 24°* | 50/5 | 91% | MC (1PB) |
ye121 | 24° | 35/0 | 100% | MEOC |
ye121 | 25° | 68/0 | 100% | MEOC |
ax161; him-8 | 16° | 18/11 | 62% | MC |
ax161; him-8 | 20° | 24/7 | 77% | MC |
ax161; him-8 | 23° | 27/0 | 100% | MEOC |
ax161; him-8 | 24° | 20/0 | 100% | MEOC |
ax161; him-8 | 25° | 25/0 | 100% | MEOC |
ax227 | 20° | 51/6 | 89% | MC (1PB) |
ax227 | 23° | 50/0 | 100% | MEOC† |
ax72 | 16° | 34/4 | 89% | MC (1PB) |
Genotype . | Temperature (°C) . | MI/MII . | %MI . | In utero embryo phenotype . |
---|---|---|---|---|
N2 | 20° | 11/9 | 55% | MC |
him-8 | 20° | 13/8 | 62% | MC |
ax212 | 20° | 14/9 | 61% | MC |
ax212 | 23° | 13/6 | 68% | MC |
ax212 | 24° | 24/7 | 77% | MC |
ax212 | 25° | 20/2 | 91% | MC (1PB) |
ye121 | 20° | 23/14 | 62% | MC |
ye121 | 23° | 45/16 | 74% | MC |
ye121 | 24°* | 50/5 | 91% | MC (1PB) |
ye121 | 24° | 35/0 | 100% | MEOC |
ye121 | 25° | 68/0 | 100% | MEOC |
ax161; him-8 | 16° | 18/11 | 62% | MC |
ax161; him-8 | 20° | 24/7 | 77% | MC |
ax161; him-8 | 23° | 27/0 | 100% | MEOC |
ax161; him-8 | 24° | 20/0 | 100% | MEOC |
ax161; him-8 | 25° | 25/0 | 100% | MEOC |
ax227 | 20° | 51/6 | 89% | MC (1PB) |
ax227 | 23° | 50/0 | 100% | MEOC† |
ax72 | 16° | 34/4 | 89% | MC (1PB) |
Hermaphrodites (10-20) of the indicated genotypes were kept at 15°C or shifted from 15°C to the indicated temperature as young L4 larvae. Unless otherwise noted, the duration of incubation was 16-20 hours prior to analysis. In all cases, mutant hermaphrodites were crossed with wildtype males to ensure that only maternal effects were scored. The embryos were fixed and stained with DAPI and α-tubulin antibodies for staging. % MI represents the number of meiosis I embryos produced divided by the total number of meiotic embryos scored. The in utero phenotype column reflects the oldest and most predominant embryonic stage produced by the treated hermaphrodites. (MC,multicellular embryos; MEOC, meiotic one-cell embryos.)
The 1PB after MC indicates that the majority of treated hermaphrodites(>50%) produced embryos containing a single polar body. MC alone implies that the 2PB Class predominates.
Blue-permissive condition (>85% hatch), Green — semi-permissive condition (2-20% hatch).
Red — restrictive condition (0% hatch).
Hermaphrodites were incubated 12 hours prior to analysis.
ax227 at 23-25°C produces 100% MEOC.