gli1 MO injection into wild-type embryos phenocopies dtr nk2.2 defects
| gli MO injected . | Very strong . | dtr phenocopy . | Partial phenocopy . | wt nk2.2 . | Total . |
|---|---|---|---|---|---|
| Control (6-13 ng) | 0 | 0 | 0 | 150 (100%) | 150 |
| 0.2-1 ng | 0 | 0 | 44 (43%) | 58 (57%) | 102 |
| 2 ng | 0 | 32 (60%) | 14 (26%) | 7 (13%) | 53 |
| 3 ng | 38 (26%) | 48 (33%) | 33 (23%) | 17 (12%) | 146 |
| 5-7 ng | 37 (52%) | 21 (30%) | 13 (18%) | 0 | 71 |
| 11 ng | 37 (76%) | 12 (24%) | 0 | 0 | 49 |
| gli MO injected . | Very strong . | dtr phenocopy . | Partial phenocopy . | wt nk2.2 . | Total . |
|---|---|---|---|---|---|
| Control (6-13 ng) | 0 | 0 | 0 | 150 (100%) | 150 |
| 0.2-1 ng | 0 | 0 | 44 (43%) | 58 (57%) | 102 |
| 2 ng | 0 | 32 (60%) | 14 (26%) | 7 (13%) | 53 |
| 3 ng | 38 (26%) | 48 (33%) | 33 (23%) | 17 (12%) | 146 |
| 5-7 ng | 37 (52%) | 21 (30%) | 13 (18%) | 0 | 71 |
| 11 ng | 37 (76%) | 12 (24%) | 0 | 0 | 49 |
Very strong: nk2.2 extremely reduced in malformed embryos.
Partial phenocopy: nk2.2 reduced but not absent.