Semi-quantitative analysis of percentage DiI-labelled nerve fibres reaching hair cells in each of the three rows of OHCs
| . | Apex . | . | . | Middle . | . | . | Base . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Row of OHC . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | ||||||
| BDNF-/- | 0.1 | 0 | 0 | 2.9 | 1.2 | 0 | 18 | 10 | 0.1 | ||||||
| BDNFNT3/NT3 | 2.6 | 0.2 | 0 | 46 | 35 | 32 | 88 | 84 | 56 | ||||||
| . | Apex . | . | . | Middle . | . | . | Base . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Row of OHC . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | ||||||
| BDNF-/- | 0.1 | 0 | 0 | 2.9 | 1.2 | 0 | 18 | 10 | 0.1 | ||||||
| BDNFNT3/NT3 | 2.6 | 0.2 | 0 | 46 | 35 | 32 | 88 | 84 | 56 | ||||||
The 8th cranial nerve was traced using DiI in the indicated genotypes and the cochleae was dissected into three portions, apex, middle and base. Confocal images were acquired and the OHCs were counted in each dissected part the cochlea. The number of nerve fibres projecting to the 1st, 2nd and 3rd row of OHCs were then determined and are presented as percentage of the number of OHCs in row one(wild type n=14; BDNFNT3/NT3 n=11;BDNF-/- n=7). In wild-type mice, traced fibres reaching the three rows of OHCs was seen throughout the longitudinal axis (i.e. 100%).