Neuronal cell counts in sensory ganglia in wild-type,BDNF–/– and BDNFNT3/NT3mice at P0
| Ganglion . | Mean number of neurons ± s.e.m. . | Percentage of control ± s.e.m. . |
|---|---|---|
| Trigeminal | ||
| Wild type | 30 183±1524 (n=6) | 100±5 |
| BDNF-/- | 27 610±985 (n=5) | 91±3 |
| BDNFNT3/NT3 | 33 013±1097 (n=5) | 109±4† |
| Nodose | ||
| Wild type | 11 604±439 (n=7) | 100±4 |
| BDNF-/- | 7 076±252 (n=4) | 61±2*** |
| BDNFNT3/NT3 | 9 315±548 (n=4) | 80±5†, ** |
| Dorsat root (L4) | ||
| Wild type | 9 045±592 (n=4) | 100±7 |
| BDNF-/- | 5 984±542 (n=4) | 66±6* |
| BDNFNT3/NT3 | 7 835±585 (n=5) | 87±6† |
| Ganglion . | Mean number of neurons ± s.e.m. . | Percentage of control ± s.e.m. . |
|---|---|---|
| Trigeminal | ||
| Wild type | 30 183±1524 (n=6) | 100±5 |
| BDNF-/- | 27 610±985 (n=5) | 91±3 |
| BDNFNT3/NT3 | 33 013±1097 (n=5) | 109±4† |
| Nodose | ||
| Wild type | 11 604±439 (n=7) | 100±4 |
| BDNF-/- | 7 076±252 (n=4) | 61±2*** |
| BDNFNT3/NT3 | 9 315±548 (n=4) | 80±5†, ** |
| Dorsat root (L4) | ||
| Wild type | 9 045±592 (n=4) | 100±7 |
| BDNF-/- | 5 984±542 (n=4) | 66±6* |
| BDNFNT3/NT3 | 7 835±585 (n=5) | 87±6† |
Cryostat sections from newborn mice of the indicated genotypes were prepared and stained with Cresyl Violet. Trigeminal ganglion neurons with a clear nucleus and nucleoli were counted in every 12th section, nodose every sixth and DRG every second section. ANOVA Fischer's†P<0.05 between BDNFNT3/NT3and BDNF-/- mice; ANOVA Fischer's *P<0.05,**P<0.005 and ***P<0.001 between mutant mice and wild-type mice.