. | . | Joint region phenotype . | . | . | . | |
---|---|---|---|---|---|---|
. | Concentration of bapx1-MO (mg/ml) . | . | . | Percentages . | . | |
. | . | Joint loss . | Joint+RAP loss . | No BH . | Small BH . | |
Uninjected | 0 (0/100) | 0 (0/100) | 0 | 0 | ||
MO1 | 1 | 51 (108/213) | 1 (2.5/213) | 0 | 16 | |
3 | 80 (147/183) | 23 (42/183) | 0 | 70 | ||
MO2 | 1 | 2 (1/48) | 0 (0/48) | 0 | 2 | |
2 | 88 (66/75) | 23 (17/75) | 48 | 52 | ||
MO1+2 | 0.5 each | 77 (51/66) | 5 (3/66) | 3 | 97 | |
1.5 each | 98 (59/60) | 55 (33/60) | 67 | 33 |
. | . | Joint region phenotype . | . | . | . | |
---|---|---|---|---|---|---|
. | Concentration of bapx1-MO (mg/ml) . | . | . | Percentages . | . | |
. | . | Joint loss . | Joint+RAP loss . | No BH . | Small BH . | |
Uninjected | 0 (0/100) | 0 (0/100) | 0 | 0 | ||
MO1 | 1 | 51 (108/213) | 1 (2.5/213) | 0 | 16 | |
3 | 80 (147/183) | 23 (42/183) | 0 | 70 | ||
MO2 | 1 | 2 (1/48) | 0 (0/48) | 0 | 2 | |
2 | 88 (66/75) | 23 (17/75) | 48 | 52 | ||
MO1+2 | 0.5 each | 77 (51/66) | 5 (3/66) | 3 | 97 | |
1.5 each | 98 (59/60) | 55 (33/60) | 67 | 33 |
Animals were fixed at 4 days and Alcian stained, then scored in wholemounts. Both left and right sides of each animal were scored, and counted as half an animal. Joint loss animals lacked the joint, but had the cartilaginous retroarticular process (RAP) of Meckel's cartilage, whereas joint + RAP loss animals lacked both the joint and RAP. No animals were observed to lack the process yet have the joint. BH, basihyal.