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Table 1.

PCR primer sets for real-time, quantative RT-PCR

PCR primersSequenceReference
Xnr3 F, 5′-CTTCTGCACTAGATTCTG-3′; R,5′-CAGCTTCTGGCCAAGACT-3′ Kofron et al., 1999 
Xnr5 F, 5′-TCACAATCCTTTCACTAGGGC-3′; R,5′-GGAACCTCTGAAAGGAAGGC-3′ Yang et al., 2002  
Xnr6 F, 5′-TCCAGTATGATCCATCTGTTGC-3′; R,5′-TTCTCGTTCCTCTTGTGCCTT-3′ Takahashi et al., 2000  
Siamois F, 5′-CTCCAGCCACCAGTACCAGATC-3′; R,5′-GGGGAGAGTGGAAAGTGGTTG-3′ Brannon et al., 1996 
Twin F, 5′-AGTCGTGCCTTTGAAGCCACT-3′; R,5′-CGCCGCTTGCATAGAAACAGT-3′ This work 
EF1a F, 5′-CAGATTGGTGCTGGATATGC-3; R, 5′-ACTGCCTTGATGACTCCTAG-3′ This work 
PCR primersSequenceReference
Xnr3 F, 5′-CTTCTGCACTAGATTCTG-3′; R,5′-CAGCTTCTGGCCAAGACT-3′ Kofron et al., 1999 
Xnr5 F, 5′-TCACAATCCTTTCACTAGGGC-3′; R,5′-GGAACCTCTGAAAGGAAGGC-3′ Yang et al., 2002  
Xnr6 F, 5′-TCCAGTATGATCCATCTGTTGC-3′; R,5′-TTCTCGTTCCTCTTGTGCCTT-3′ Takahashi et al., 2000  
Siamois F, 5′-CTCCAGCCACCAGTACCAGATC-3′; R,5′-GGGGAGAGTGGAAAGTGGTTG-3′ Brannon et al., 1996 
Twin F, 5′-AGTCGTGCCTTTGAAGCCACT-3′; R,5′-CGCCGCTTGCATAGAAACAGT-3′ This work 
EF1a F, 5′-CAGATTGGTGCTGGATATGC-3; R, 5′-ACTGCCTTGATGACTCCTAG-3′ This work 
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