Primer sets used for PCR analysis
| Target gene . | Upstream primer (5′ to 3′) . | Downstream primer (5′ to 3′) . | Cycle number . | Number of bp . | Accession number/reference . |
|---|---|---|---|---|---|
| ET | CCT ATC CTT GAC TTG CTA CA | GTT TTG GGG AAG GAG GGT AT | 24 | 255 | AF173940 |
| Pax6 | GCA ACC TGG CGA GCG ATA AGC | CCT GCC GTC TCT GGT TCC GTA GTT | 28 | 450 | U76386 |
| Six3 | TTG TCT GTC TGT CTC TTG TT | TTC TGT GTT TGG TTT ATC TC | 30 | 369 | AF183571 |
| Rx1 | CCC CAA CAG GAG CAT TTA GAA GAC | AGG GCA CTC ATG GCA GAA GGT T | 28 | 416 | AF017273 |
| tll | ACT TGC CTC TCG TGC TGC TCT ACT G | ATC CGG TCG GGT TGC TCA TCT T | 30 | 351 | U67886 |
| Lhx2 | ACC CTC CTC CCC CAT TAC TCA C | AGG GCA TAT CTG GGC ATC TTC A | 30 | 461 | AY141037 |
| Optx2 | ACA GAG CAG CGG CGG CAA AGA | GAG CGC TCC CTG GTA CTG TGA CTG A | 30 | 296 | AF081352 |
| NCAM | CAC AGT TCC ACC AAA TGC | GGA ATC AAG CGG TAC AGA | 30 | 343 | Xenbase |
| Otx2 | GGA TGG ATT TGT TAC ATC CGT C | CAC TCT CCG AGC TCA CTT CCC | 25 | 315 | U19813 |
| XAG | GAC TGG TGC TGT TCA ACC TTG | CAT TGG GAA ATA ACT GGG ACC | 25 | 349 | U76752 |
| H4 | CGG GAT AAC ATT CAG GGT ATC ACT | ATC CAT GGC GGT AAC TGT CTT CCT | 24 | 189 | Hollemann et al., 1998 |
| Target gene . | Upstream primer (5′ to 3′) . | Downstream primer (5′ to 3′) . | Cycle number . | Number of bp . | Accession number/reference . |
|---|---|---|---|---|---|
| ET | CCT ATC CTT GAC TTG CTA CA | GTT TTG GGG AAG GAG GGT AT | 24 | 255 | AF173940 |
| Pax6 | GCA ACC TGG CGA GCG ATA AGC | CCT GCC GTC TCT GGT TCC GTA GTT | 28 | 450 | U76386 |
| Six3 | TTG TCT GTC TGT CTC TTG TT | TTC TGT GTT TGG TTT ATC TC | 30 | 369 | AF183571 |
| Rx1 | CCC CAA CAG GAG CAT TTA GAA GAC | AGG GCA CTC ATG GCA GAA GGT T | 28 | 416 | AF017273 |
| tll | ACT TGC CTC TCG TGC TGC TCT ACT G | ATC CGG TCG GGT TGC TCA TCT T | 30 | 351 | U67886 |
| Lhx2 | ACC CTC CTC CCC CAT TAC TCA C | AGG GCA TAT CTG GGC ATC TTC A | 30 | 461 | AY141037 |
| Optx2 | ACA GAG CAG CGG CGG CAA AGA | GAG CGC TCC CTG GTA CTG TGA CTG A | 30 | 296 | AF081352 |
| NCAM | CAC AGT TCC ACC AAA TGC | GGA ATC AAG CGG TAC AGA | 30 | 343 | Xenbase |
| Otx2 | GGA TGG ATT TGT TAC ATC CGT C | CAC TCT CCG AGC TCA CTT CCC | 25 | 315 | U19813 |
| XAG | GAC TGG TGC TGT TCA ACC TTG | CAT TGG GAA ATA ACT GGG ACC | 25 | 349 | U76752 |
| H4 | CGG GAT AAC ATT CAG GGT ATC ACT | ATC CAT GGC GGT AAC TGT CTT CCT | 24 | 189 | Hollemann et al., 1998 |
Primer sets were designed from the indicated GenBank sequence or from the indicated source (see Materials and methods for the details of the PCR reaction conditions). Xenbase primer sequences can be found at http://www.xenbase.org/