Distribution of X-gal-positive cells in blastocysts after labeling single blastomeres of two-cell or four-cell stage embryos
| Injection stage . | Injected . | Blue . | Normal . | Localized . |
|---|---|---|---|---|
| Two cell | 278 | 178 | 147 | 107 (73%) |
| Four cell | 172 | 23 | 23 | 22 (96%) |
| Injection stage . | Injected . | Blue . | Normal . | Localized . |
|---|---|---|---|---|
| Two cell | 278 | 178 | 147 | 107 (73%) |
| Four cell | 172 | 23 | 23 | 22 (96%) |
Embryos were cultured in vitro after the injection with pCAG-Cre and the blastocysts were stained. Most X-gal-positive embryos (No. blue) were morphologically normal (normal). 73% (injection at the two-cell stage) and 96%(injection at the four-cell stage) of embryos showed localized distribution of labeled cells.